Protective Effects and Mechanisms of 4,4'-diisothiocyanostibibene-2,2'-disulfonic Acid Injected by Intarcerebroventricular against Cerebral Ischemia-reperfusion Injury Rat

doi:10.3969/j.issn.1674-5817.2015.02.002

Abstract

Abstract: Objective To explore the protective effects and mechanisms of 4,4'-diisothiocyanostibibene-2,2'-disulfonic acid (DIDS) injected by intarcerebroventricular against cerebral ischemia-reperfusion injury (CIRI) in middle cerebral artery occlusive (MCAO) rats. Methods Focal cerebral ischemia in rats was produced by 2 h occlusion of the middle cerebral artery and 24 h reperfusion. DIDS and normal saline (NS) was injected by intarcerebroventricular. The neurologic deficit scores were investigated according to Zea-Longa's Standard. The infarct areas were assessed with software Imagepro Plus 6.0 after TTC staining. The levels of Bcl-2 and Caspase-3 in central neutral system were assessed by western blot. And the activities of inducible nitric oxide synthase (iNOS) as well as glutathione peroxidase (GSH-PX) were determined by specific kit. Results DIDS (100 μmol/L) could decrease the neurologic deficit score, reduced the infarct areas of the brain in CIRI rats, enhance the expression of Bcl-2, depresse the expression of Caspase-3, and decrease the activity of iNOS. Conclusion DIDS has a protective effect on CIRI of rat and this effect is related to enhancing the expression of Bcl-2 and diminishing Caspase-3 expression, decreasing the activity of iNOS.

Key words: Cerebral ischemia-reperfusion injury (CIRI), Intracerebroventricular injection, DIDS, Bcl-2, Caspase-3, Inducible nitric oxide (iNOS) synthase, Glutathione peroxidase (GSH-PX)

CLC Number:

Q95-33

YUAN Zheng, ZHANG Lu, WU Wen-qing, YUAN Zi-yan, ZHAO Shan-min, YU Chen-lin, LIN Li-fang, TANG Qiu. Protective Effects and Mechanisms of 4,4'-diisothiocyanostibibene-2,2'-disulfonic Acid Injected by Intarcerebroventricular against Cerebral Ischemia-reperfusion Injury Rat[J]. Laboratory Animal and Comparative Medicine, 2015, 35(2): 92-96.

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