Laboratory Animal and Comparative Medicine ›› 2014, Vol. 34 ›› Issue (6): 438-442.DOI: 10.3969/j.issn.1674-5817.2014.06.002

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Comparison of Expression Level of SLC24A5, SLC39A9 Genes in Cricetulus griseus and the Albino mutant

LIU Hui-fang1,2, SUN Shu-fang3, ZENG Lin1, CAO Yu-hua2, YAN Xiang2, FAN jun-wen2, SUN Zhao-zeng2, WANG Xin1   

  1. 1. Qingdao Agriculture University, Qingdao 266109,China;
    2. Laboratory Animal Center of the Academy of Military Medical Science, Beijing 100071, China;
    3. Qingdao Blood Bank, Qingdao 266071, China
  • Received:2014-05-22 Online:2014-12-25 Published:2014-12-25

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Abstract: Objective To investigate the expression level of the soluble material transport protein SLC24A5 (solute carrier family 24, member 5, SLC24A5) and SLC39A9 (solute carrier family 39, member 9, SLC39A9) between the Cricetulus griseus and its albino mutation strain, A:CHA, and analyze the function on the albino phenotype. Methods The total mRNA of skin tissue in the Cricetulus griseus and A:CHA was extacted. The PCR method was used to select primers. The expression levels of SLC24A5, SLC39A9 was analyzed by real time PCR. Results The ratio of expression level of SLC24A5, SLC39A9 between Cricetulus griseus and A:CHA were 2 and 3.8 times. Conclusion The gene expression level of SLC24A5, SLC39A9 in the A:CHA were lower than that in the Cricetulus griseus. The study strongly supports the notion that the two genes may be contribute to the albino phenotype.

Key words: Cricetulus griseus, A:CHA, SLC24A5, SLC39A9, Real time quantitative PCR

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