Compensatory Mechanism May Exist between SUMF2 and SUMF1 to Keep Sulfatase Activity Unchanged

doi:10.3969/j.issn.1674-5817.2014.05.002

Abstract

Abstract: Objective To investigate the function of SUMF2 in molecular level, cell level, tissue level and the relationship with sulfatases activity though Sumf2 knockout mice. Methods Using confocol microscopy to observe mice SUMF2 cell location; using cells and tissue proteins react with 4 sulfatases substrates and detect the products generation to count the 4 type sulfatases activity; using MTT method to test the proliferation of mouse embryonic fibroblast cells (MEFs); using Alcian blue staining to observe whether the substrates of sulfatases stored in the tissue samples; using qPCR method to test the expression of Sumf1. Results Mice SUMF2 is located in the endoplasmic reticulum; four sulfatases activity were slight reduced in Sumf2^-/- mice, and proliferation of mouse embryonic fibroblasts were slightly attenuated, but do not reach significant level. There is no obviously distinguish of hematoxylin and eosin (HE) and glycosaminoglycans (GAGs) staining in lung, liver, kidney and skin; but the expression of Sumf1 was down regulated in Sumf2^-/- mice. Conclusion Sumf2 was localized in the endoplasmic reticulum, loss of SUMF2 in vivo compensatory down-regulation the expression of Sumf1 so that the sulfatase activity remained unchanged.

Key words: Sumf2, Sumf1, Sulfatases activity, Eendoplasmic reticulum

CLC Number:

Q95-33

ZHOU Tao, ZHANG Hong-xin, ZHU Hou-bao, TANG Ling-yun, KUANG Fang-zhe, WANG Zhu-gang. Compensatory Mechanism May Exist between SUMF2 and SUMF1 to Keep Sulfatase Activity Unchanged[J]. Laboratory Animal and Comparative Medicine, 2014, 34(5): 353-359.

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