Intracytoplasmic Sperm Injection and Transposon-mediated Transgenic Technology

doi:10.3969/j.issn.1674-5817.2013.04.015

Abstract

Abstract: Intracytoplasmic sperm injection (ICSI) has been widely used for generating offspring in human infertility clinics and in reproductive researched on mice. Many researchers engaged in animal transgenesis still consider it somewhat cumbersome. However, the greatest advantage of ICSI-mediated transgenesis is that it allows introduction of very large DNA transgenes (e.g., yeast artificial chromosomes), with relatively high efficiency into the genomes of hosts, as compared to pronuclear injection. Recently, researchers have developed an active form of intracytoplasmic sperm injection-mediated transgenesis (ICSI-Tr) with fresh sperm utilizing transposons. The transgenic efficiencies rival all transgenic techniques except that of lentiviral methods so that it is worthy of promotion and application in animal transgenic research.

Key words: ICSI, Transposon, Transgenesis

CLC Number:

Q95-33

KONG Peng-cheng, WANG Mei-shan, ZHU Lian, LI He-ping, JIANG Man-xi, CHEN Xue-jin. Intracytoplasmic Sperm Injection and Transposon-mediated Transgenic Technology[J]. Laboratory Animal and Comparative Medicine, 2013, 33(4): 319-323.

References

[1] Gordon JW, Scangos GA, Plotkin DJ, et al.Genetic transformation of mouse embryos by microinjection of purified DNA[J]. Proc Natl Acad Sci USA, 1980, 77(12):7380-7384.
[2] Hofmann A, Kessler B, Ewerling S, et al.Efficient transgenesis in farm animals by lentiviral vectors[J]. EMBO Rep, 2003, 4(11):1054-1060.
[3] Gertsenstein M, Lobe C, Nagy A.ES cell-mediated conditional transgenesis[J]. Methods Mol Biol, 2002, 185:285-307.
[4] Lavitrano M, Camaioni A, Fazio VM, et al.Sperm cells as vectors for introducing foreign DNA into eggs: genetic transformation of mice[J]. Cell, 1989, 57(5):717-723.
[5] Ding S, Wu X, Li G, et al.Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice[J]. Cell, 2005, 122(3):473-483.
[6] Gordon JW and Ruddle FH. Integration and stable germ line transmission of genes injected into mouse pronuclei[J]. Science, 1981, 214(4526):1244-1246.
[7] Hammer RE, Pursel VG, Rexroad CE Jr, et al.Production of transgenic rabbits, sheep and pigs by microinjection[J]. Nature, 1985, 315(6021):680-683.
[8] Perry AC.Hijacking oocyte DNA repair machinery in transgenesis?[J]. Mol Reprod Dev, 2000, 56(2 Suppl):319-324.
[9] Muller U.Ten years of gene targeting: targeted mouse mutants, from vector design to phenotype analysis[J]. Mech Dev, 1999, 82(1-2):3-21.
[10] Wall RJ.Pronuclear microinjection[J]. Cloning Stem Cells, 2001, 3(4):209-220.
[11] Nakanishi T, Kuroiwa A, Yamada S, et al.FISH analysis of 142 EGFP transgene integration sites into the mouse genome[J]. Genomics, 2002, 80(6):564-574.
[12] Lewis-Williams J, Harvey M, Wilburn B, et al.Analysis of transgenic mosaicism in microinjected mouse embryos using fluorescence in situ hybridization at various developmental time points[J]. Theriogenology, 1996, 45(1):335.
[13] Feitosa WB, Mendes CM, Milazzotto MP, et al.Exogenous DNA uptake by bovine spermatozoa does not induce DNA fragmentation[J]. Theriogenology, 2010, 74(4):563-568.
[14] Lavitrano M, Forni M, Varzi V, et al.Sperm-mediated gene transfer: production of pigs transgenic for a human regulator of complement activation[J]. Transplant Proc, 1997, 29(8):3508-3509.
[15] Lavitrano M, Stoppacciaro A, Bacci ML, et al.Human decay accelerating factor transgenic pigs for xenotransplantation obtained by sperm-mediated gene transfer[J]. Transplant Proc, 1999, 31(1-2):972-974.
[16] Perry AC, Wakayama T, Kishikawa H, et al.Mammalian transgenesis by intracytoplasmic sperm injection[J]. Science, 1999, 284(5417):1180-1183.
[17] Yanagimachi R.Intracytoplasmic injection of spermatozoa and spermatogenic cells: its biology and applications in humans and animals[J]. Reprod Biomed Online, 2005, 10(2):247-288.
[18] Morozumi K, Shikano T, Miyazaki S, et al.Simultaneous removal of sperm plasma membrane and acrosome before intracytoplasmic sperm injection improves oocyte activation/embryonic development[J]. Proc Natl Acad Sci USA, 2006, 103(47):17661-17666.
[19] Morozumi K and Yanagimachi R. Incorporation of the acrosome into the oocyte during intracytoplasmic sperm injection could be potentially hazardous to embryo development[J]. Proc Natl Acad Sci USA, 2005, 102(40):14209-14214.
[20] Yamauchi Y, Yanagimachi R and Horiuchi T. Full-term development of golden hamster oocytes following intracytoplasmic sperm head injection[J]. Biol Reprod, 2002, 67(2):534-539.
[21] Azerędo G, Esper C and Resende K. Evaluation of plasma membrane integrity of frozen-thawed goat spermatozoa with or without seminal plasma[J]. Small Ruminant Research, 2001, 41(3):257-263.
[22] Moisyadi S, Kaminski JM and Yanagimachi R. Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals[J]. Comp Immunol Microbiol Infect Dis, 2009, 32(2):47-60.
[23] Li C, Mizutani E, Ono T, et al.An efficient method for generating transgenic mice using NaOH-treated spermatozoa[J]. Biol Reprod, 2010, 82(2):331-340.
[24] Moreira PN, Giraldo P, Cozar P, et al.Efficient generation of transgenic mice with intact yeast artificial chromosomes by intracytoplasmic sperm injection[J]. Biol Reprod, 2004, 71(6):1943-1947.
[25] Osada T, Toyoda A, Moisyadi S, et al.Production of inbred and hybrid transgenic mice carrying large (> 200 kb) foreign DNA fragments by intracytoplasmic sperm injection[J]. Mol Reprod Dev, 2005, 72(3):329-335.
[26] Perry AC, Rothman A, de las Heras JI, et al. Efficient metaphase II transgenesis with different transgene archetypes[J]. Nat Biotechnol, 2001, 19(11):1071-1073.
[27] Lois C, Hong EJ, Pease S, et al.Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors[J]. Sci, 2002, 295(5556):868-872.
[28] Szczygiel MA, Moisyadi S and Ward WS. Expression of foreign DNA is associated with paternal chromosome degradation in intracytoplasmic sperm injection-mediated transgenesis in the mouse[J]. Biol Reprod, 2003, 68(5):1903-1910.
[29] Suganuma R, Pelczar P, Spetz JF, et al.Tn5 transposase-mediated mouse transgenesis[J]. Biol Reprod, 2005, 73(6):1157-1163.
[30] Li X, Lobo N, Bauser CA, et al.The minimum internal and external sequence requirements for transposition of the eukaryotic transformation vector piggyBac[J]. Mol Genet Genomics, 2001, 266(2):190-198.
[31] Wu S, Ying G, Wu Q, et al.Toward simpler and faster genome-wide mutagenesis in mice[J]. Nat Genet, 2007, 39(7):922-930.
[32] Suster ML, Sumiyama K and Kawakami K. Transposon-mediated BAC transgenesis in zebrafish and mice[J]. BMC Genomics, 2009, 10:477.
[33] Rostovskaya M, Naumann R, Fu J, et al.Transposon mediated BAC transgenesis via pronuclear injection of mouse zygotes[J]. Genesis, 2013, 51(2):135-141.
[34] Nakanishi H, Higuchi Y, Kawakami S, et al.piggyBac transposon-mediated long-term gene expression in mice[J]. Mol Ther, 2010, 18(4):707-714.
[35] Shinohara ET, Kaminski JM, Segal DJ, et al.Active integration: new strategies for transgenesis[J]. Transgenic Res, 2007, 16(3):333-339.
[36] Brinster RL, Chen HY, Trumbauer ME, et al.Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs[J]. Proc Natl Acad Sci USA, 1985, 82(13):4438-4442.