Laboratory Animal and Comparative Medicine ›› 2011, Vol. 31 ›› Issue (1): 38-42.DOI: 10.3969/j.issn.1674-5817.2011.01.008

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Efficient Repeated Low-dose Intravaginal Infection with SHIV1157ipd3N4 in Rhesus Monkey

CONG Zhe1, JIANG Hong1, JIN Guang1, WANG Wei1, GAO Hong1, YAO Nan1, TAO Zhen1, CHEN Ting1, YANG Zhi-wei1, Sylvain. Fleury2, Anick Chalifour2, Ruth. M.Ruprecht3, WEI Qiang1, QIN Chuan1   

  1. 1. Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese medicine,Beijing 100021, China;
    2. Dana-Farber Cancer Institute, Boston, Massachusetts 021151; Harvard Medical School,Boston, Massachusetts 021152;
    3. Mymetics Corporation, CH 1066 Epalinges, Switzerland
  • Received:2010-07-26 Online:2011-02-25 Published:2011-02-25

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Abstract: Objective To establish a CCR5-specific chimeric simian/human immunodeficiency virus, SHIV1157ipd3N4 repeated low-dose intravaginal infection model with rhesus monkeys. This new chimeric model could hopefully be more efficient model in AIDS vaccine development. Methods Six female Chinese rhesus monkeys were enrolled in this study. All animals were intra-vaginally challenged 11 times with 1 ml of a phosphate-buffer viral solution containing the heterologous SHIV1157ipd3N4, with 20 TCID50 for the first 3 challenges and 10 TCID50 for the remaining 8 challenges. Each challenge was accomplished at the interval of 4-7 days. Whole blood was collected and plasma virus was quantified by real-time SYBR Green RT-PCR and T cell subsets were determined by flow cytometry analysis. Results The systematic infection of six rhesus monkeys was established successfully after 11 challenges. The peaks of viral loads were between 106copies/ml to 108copies/ml.Conclusion The repeated low-dose technique was used for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus monkey. This model aliking sexual route would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.

Key words: SHIV1157ipd3N4, Vaginal transmission, Chinese rhesus monkey

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