Abstract: Objective To establish the sulfatase modifying factor 2 (Sumf2) gene conditional knock-out (CKO) mouse model and to analyze the biological functions for further in vivo study. Methods Use the technology of bioinformatics, ET cloning, homologous recombination, and microinjection, etc to establish the CKO mouse model. Mating transgenic (TG) homozygous EIIa-cre males with CKO heterozygous females, Sumf2 gene knock out (KO) mouse model was established. The deletion of Sumf2 gene in the KO mouse was confirmed by PCR, RT-PCR and western-blot analysis. Results There are about 20% - 19 ES cells positive clones, 2 chimeras males and about 57% of 28 Aguoti mice-16 heterozygous obtained. Based on this result, mating TG homozygous EIIa-cre males and CKO heterozygous females, Sumf2 gene KO mouse model has been established. Analyzed by PCR, RT-PCR and Western Blot, it is verified for the deletion of Sumf2 gene at different levels. Preliminarily phenotypic observations show that the growth and development of Sumf2 KO mice were generally normal, and abnormalities were not found from the data of routine blood tests and some of serum biochemical tests in comparison with those of wild type mice. Conclusions The establishment of Sumf2 CKO mouse model and Sumf2 KO mouse model has paved the way for further study of the functions of this gene.

Key words: Sumf2 gene, Conditional knockout mice, Phenotype analysis