Abstract: The high concentration ethylene glycol （EG-8，8 mol/L） and galactose（1.7mol/L） were used as a cryoprotectant and thawing diluents for embryo cryopreservation by vitrification in MMTV-Wnt-1 transgenic mice. The best results of cryopreservation were obtained if those embryos kept balance in EG-2 （2mol/L） for 4～6 minute before freeze and thawing 3～5 minute in galactose. The percentage of embryos cryopreserved and retrieve is 88. 61% （109/123）； there is 88. 99%（97/109）of these embryos develop and 81. 65%（89/109）developing to hatch blastocysts. After these development embryos were transfer to pseudocyesis mouse, 38. 36%（69/181） developed to live young, and a half of these is positive mouse on the PCR test. It follows that high concentration ethylene glycol and galactose as a cryoprotectant for cryopreseveration by vitrification is effective.

Key words: Ethylene glycol, Transgenic mice, Embryo cryopreservation