Abstract: The authors had isolated skeletal muscle satellite cells by mechanic and enzymic digestion,purified them by Pecoll discontinuous density gradient centrifugation and identified them immunocytochemically by Desmin-labeling,and multiplied the satellite cells by administration of promoting heptocyte growth factor （pHGF）. The purity of harvested satellite cells exceeded 90%.It was suggested that the pHGF was usful for prompting satellite cell growth by shortening the lag period and enhancing their proliferation capacities.

Key words: Skeletal muscle satellite cell, Isolation, Culture, Multiplication