Abstract: "Four Macaca speciosa without infected HBV naturally were selected for this study. Two of them used as HBV infection, another Two for the contra 1. The serum of animals were drawn once for every 2 to 4 weeks in the first year, and for every 2 to 4 months in the second year. The positive of SGPT, HBV markers, HBV-DNA, Dane particles were observed by R-F, RPHA, ELISA, SPRIA, Hybridization spot test and EM, respectively. The HBsAg-positive lasted for 52( 1#）and 53(8.) weeks. Performed liver biopsy in various period, and spccimcnts were observed for HBsAg, HBeAg and HBV-DNA by light microscope, EM, histochemistry, immuno-histochemistry and in situ hybridization. The results showed that the tested animals developed into acute to chronic hepatitis B and their features resemble that in human. We also inoculated sera from 1* and 8* into 13* which the SGPT and HBsAg positive stronger at 28 weeks, but it died for dysentery at 8 th days, so that observation stopped. "

Key words: "HBV, Experimental infection, Macaca speciosa, Hybridization Spot, In situ Hybridization, Immunochemistry, EM. "