Abstract:

Objective To explore the establishment of a sheep model of peripheral cannulation cardiopulmonary bypass and the key points of peri-CPB management. Methods Eight healthy adult sheep weighing 70-80 kg were selected. After induction of general anesthesia and endotracheal intubation, cardiopulmonary bypass was established via the left jugular vein and carotid artery, with continuous perfusion at a flow rate of 60-80 ml/(kg·min). The modeling time, cannulation success rate, intraoperative blood loss and adverse events during CPB were recorded. Mean arterial pressure (MAP), central venous pressure (CVP), arterial partial pressure of oxygen (PO₂), partial pressure of carbonF dioxide (PCO₂), hematocrit (HCT), serum potassium, serum calcium and lactate (Lac) were collected before operation (T1), 30 minutes after the start of CPB (T2) and at the end of operation (T3). SPSS 25.0 statistical software was used for analysis. Measurement data were expressed as x±s, and paired-samples t-test was performed. P<0.05 was considered statistically significant. Results All 8 sheep successfully established the cardiopulmonary bypass model, with a 100% modeling success rate, no adverse events or death during the operation. There were no significant differences in all indicators between T3 and T1 (P>0.05). Compared with T1, MAP, CVP, HCT and serum calcium were significantly decreased at T2 (P<0.05), while lactate was significantly increased (P<0.05). There were no significant differences in PO₂, PCO₂ or serum potassium (P>0.05). Conclusion The establishment of a peripheral cannulation CPB model in sheep, combined with standardized peri-CPB management and modified catheters to address insufficient cerebral perfusion can ensure a high modeling success rate. The model has stable circulation and strong reproducibility, and can provide reliable support for experimental research related to minimally invasive cardiovascular surgery.

Key words: Cardiopulmonary bypass, Sheep model, Peripheral Kannulation, Perioperative management