Table of Content

25 October 1998, Volume 18 Issue 3,4

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Study on Polymorphism of Biochemical Markers in Zmu-1：DHP Guinea Pig

LIU Di-Wen, GUO Han-Shen, FU Jun, ZHANG Shui-Li, HU Gen-Lin

1998, 18(3,4):  129-132. 

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To study the genetic characteristics of Zmu-1：DHP guinea pig,14 biochemical markers were analysed by electrophoretic method,and compared with those of the parent DHP strain. The results reveals that no polymorphism is showed on 10 loci in Zmu-1：DHP strain and 9 loci in DHP strain. Both strains manifest polymorphism on G-3-PD，G-6-PD,Es-6 and Es-8 loci，and GOT-1 locus in DHP strain is also polymorphic. There are a and b alleles on above 5 polymorphic loci and beside there is c allele on Es-8 locus in DHP strain.It also shows that the genotype on G-6-PD locus is sex related，most male guinea pigs possess b allele on G-6-PD locus while most females possess a allele on the same locus.

Study of Parthenogenetic Activation of Rabbit Eggs by Electric Stimulation

LI Jin-Song, LIU Hui, WANG Min-Kang, LIAN Li, LI Hou-Da, CHEN Da-Yuan

1998, 18(3,4):  133-136. 

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Three factors ,cytochalasin B （CB） pre-treatment,width of two electrodes and field strength of electric pulse （direct current）related to the parthenogenetic activation of rabbit eggs by electric stimulation were studied for nuclear transplantation. The results showed that the treatment of oocytes with 7.5 mg/L CB for 1 h followed by activation with electrical stimulation significantly improved the oocyte activation rate as compared with the oocytes without CB pre-treatment （79.4% vs 48. 1%，P＜0.05）. The activation rate of oocytes which were placed between the two electrodes with 0. 50mm apart was signifi-cantly improved as compared with the two electrodes with 0.25 or 1.00mm apart （79.4% vs 63. 1% or 35.0%,P＜0.05）. Furthermore,a high activation rate（90.9%）and develop-ment rate （47.5%） were achieved when oocytes were activated electrically with a field strength of 3.0 kV/cm（50μs） versus 1. 8,2.4,3.6 kV/cm.

Growth-promoting Effects of a Fructo-oligosaccharide Preparation on Bifidobacterium in Mice

SU Wen-Jin, ZHENG Zhong-Hui, LIU Rong, CHEN Fu, HUANG Yao-Jian, SONG Si-Yang

1998, 18(3,4):  137-140. 

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The growth-promoting effect of a fructo-oligosaccharide preparation （FOS） on Bi-fidobacteriumin vivo was studied in BALB/c mice. The BALB/c mice were given ig with 0,5，or 50mg FOS daily for 21 days respectively. From the 7th day the flora of B.adoles-centis in the faeces of BALB/c mice given with FOS 50 mg/d were found to be significant-ly higher than those of the mice given with FOS 0 or 5 mg/d and maintained at such lev-els. They were not diminished immediately after the administration period. Besides, the flora of B. adolescentis in the faeces of BALB/c mice reared in O³ atmospheric environment were diminished，but improved significantly when the mice were given ig with FOS 50 mg/d. The results indicated that FOS could promote the growth of Bifidobacterium in vi-vo.

Comparative Anatomy of Cerebral Vasculature in Mongolian Gerbil，Hamster and Rat

NIE Jin-Rong, NIE Chao-Hui

1998, 18(3,4):  141-143. 

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The anatomy of cerebral vasculature of Mongolian gerbils was compared with those of hamsters and Sprague-Dawley rats through vascular casting method. The results verified the unique anatomical characteristics of cerebral vasculature of the mongolian gerbil that is lack of posterior communicating arteries connecting carotid and vertebro-basilar systems, resulting in an incomplete circle of Willis.

Influence of Relative Humidity on Methacholine-induced Airway Reactivity in Sensitized Mice

SHAO You-Guang-1, ZHANG Wei-Ping-2, SONG Jian-Hui-1, GUO Han-Shen-1, WEI 尔Qing-2

1998, 18(3,4):  144-146. 

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The relationship between the relative air humidity （RH） and methacholine （MCH）-induced airway reactivity was studied in sedated and ovalbumin-sensitized mice. The re-sults showed that the wheezing symptoms induced by MCH in lower RH groups were more severe than those in the higher RH groups. It is suggested that the air RH at 55%-75% is more suitable for this testing.

Patterns of G-，C-band and Ag-NORs in Zmu-1：DHP Guinea Pig

HU Gen-Lin, GUO Han-Shen, LIU Di-Wen, FU Jun

1998, 18(3,4):  147-149. 

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The patterns of G-，C-band and Ag-NORs in Zmu-1：DHP guinea pig were observed. The results showed that the G-banding pattern was varied in the numer and position as compared with those in other stocks of guinea pig. The C-banding pattern was similar to those in other mammals. The Ag-NORs were located on the terminals of the short arms,and were 8 in number in most marrow cells. The results suggested that the chromosome karyotype of Zmu-1：DHP guinea pig has varied from those of other stocks of guinea pig.

Observations on Intestinal Flagellates of Laboratory Rat under Light Microscope and Scanning Electron Microscope

FU Jie-1, LIU Xiong-Wei-1, LU Guo-Ping-2, GAO Cheng-2

1998, 18(3,4):  150-152. 

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The five common intestinal flagellates in laboratory rats,Retortamonas sp.，Penta tri-chomonas hominis,Tetratrichamonas microti,Tritrichomoyias minuta and Tririchomonas muris were observed under light microscope and electron microscope. The movement,the shape and the organelle systems such as flagellum,costa,undulating membrance，axostyle and nucleus of each species under the light microscope were described.On the basis of morphology,Pentatrichomonas hominis and Tetratrichomonas microtiwere classified into hominis-type flagellate；Tritrichomonas minuta and Tririchomonas muris were classified into muris-type flagellate.Through the scanning electron microscope，it was observed that three flagella of Pentatrichomonas hominis were arisen from three different anterior parts of the trophozoite，that differed from the traditional morphological acknowle-dgement.

Protective Effects of Jian-Xue-Bao against CCl₄-Hepatotoxicity in Rats

WANG Wei-Zhong, HUANG Jian-Qin, JIANG Yan-Qiu, HUANG Cheng

1998, 18(3,4):  153-156. 

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The protective effects of Jian-Xue-Bao （made of Spirulina powder） against CC14-induced hepatotoxicity were studied in male SD rats weighed 180 ±13g. The results showed：①in the acute CC1₄-toxicity experiment,Jian-Xue-Bao added to diet for one month could partly protect the rat liver expressed by marked decrease of serum level of ALT，②in the experiment of liver cirrhosis induced by CCl4，Jian-Xue-Bao could partly protect the rat liver expressed by the significant reduction of serum level of laminin and the marked improvement of the pathological change in the pattern of serum amino acids and liver cirrhosis.It is suggested that Jian-Xue-Bao may have the protective effects against CC14-hepatotoxicity.