Effects of Sugar Concentration Control on the Longevity and the Mid-gut Stem Cells of female Drosophila Melanogaster

doi:10.3969/j.issn.1674-5817.2019.02.009

Abstract

Abstract: Objective To assess the effects of sugar concentration control on the longevity and the mid-gut stem cells of female Drosophila Melanogaster and then to find a optimum eating habits for women. Methods Detect the variance of body weight, survival rate, longevity and mid-gut stem cell’s form with gradient sugar concentration treatment (the sugar concentration in normal medium as 100%). Results With the increase of the age, all the fly groups’ body weight raised slowly first and then rapidly after 21 days. Body weight in the high sugar concentration treatment group increased faster than that of low sugar concentration treatment group. With the experiments of gradient sugar concentration treatment after high sugar concentration treatment, the optimum sugar concentration is 50%. With the extended of the treat time, the survival rate increased rapidly after 14 days. All the groups’ survival rates were increasing with the gradient sugar concentration treatment after high sugar concentration treatment group. Drosphila in the 50% sugar concentration treatment group had the highest survival rate. With the sugar concentration control, all the fly groups’ average longevity raised and the optimum sugar concentration was 50%. With the extended of the treat time, all the groups’ mid-gut stem cell had large morphological changes. They had be rescued by the sugar concentration control and the optimum sugar concentration was 50%. Conclusion The sugar was a important nutrient for D.melanogaster. It had large effects on D. melanogaster’s body weight, longevity and mid-gut stem cell. The body weight decreased and the longevity increased with the diet control. And they could rescue the damage on mid-gut stem cell.

Key words: Drosophila Melanogaster, Body weight, Longevity, Mid-gut stem cells

CLC Number:

Q95-33

TANG Run-dong, SONG Si-yuan, WU Wei. Effects of Sugar Concentration Control on the Longevity and the Mid-gut Stem Cells of female Drosophila Melanogaster[J]. Laboratory Animal and Comparative Medicine, 2019, 39(2): 118-123.

References

[1] Musselman LP, Fink JL, Narzinski K, et al.A high-sugar diet produces obesity and insulin resistance in wild-type Drosophila[J]. Dis Model Mech, 2011, 4(6):842-849.
[2] Na J, Musselman LP, Pendse J, et al.A Drosophila model of high sugar diet induced cardiomyopathy[J]. PLoS Genet, 2013, 9(1):1-14.
[3] Kemppainen E, George J, Garipler G, et al.Mitochondrial dysfunction plus high-sugar diet provokes a metabolic crisis that inhibits growth[J]. PLoS One, 2016, 11(1):1-28.
[4] Symonenko AV, Roshina NV, Krementsova AV, et al.Reduced neuronal transcription of escargot, the Drosophila gene encoding a snail- type transcription factor, promotes longevity[J]. Front Genet, 2018, 9:1-15.
[5] Obata F, Fons CO, Gould AP.Early-life exposure to low-dose oxidants can increase longevity via microbiome remodelling in Drosophila[J]. Nat Commun, 2018, 9(1):975.
[6] Joos JP, Saadatmand AR, Schnabel C, et al.Ectopic expression of S28A-mutated histone H3 modulates longevity, stress resistance and cardiac function in Drosophila[J]. Sci Rep, 2018, 8(1):2940.
[7] Gendron CM, Pletcher SD.MicroRNAs mir-184 and let-7 alter Drosophila metabolism and longevity[J]. Aging Cell, 2017,16(6): 1434-1438.
[8] Dobson AJ, He X, Blanc E, et al.Tissue-specific transcriptome profiling of Drosophila reveals roles for GATA transcription factors in longevity by dietary restriction[J]. NPJ Aging Mech Dis, 2018, 4:5.
[9] Staats S, Wagner AE, Kowalewski B, et al.Dietary resveratrol does not affect life span, body composition, stress response, and longevity-related gene expression in Drosophila melanogaster[J]. Int J Mol Sci, 2018, 19(1):223.
[10] Garschall K, Dellago H, Gáliková M, et al.Erratum: Ubiquitous over-expression of the DNA repair factor dPrp19 reduces DNA damage and extends Drosophila life span[J]. NPJ Aging Mech Dis, 2017, 3:10.
[11] 田祯祥. 乙醇对白砂糖依赖的雌性黑腹果蝇寿命内稳态的影响研究[J]. 四川体育科学, 2015, 2(1):38-44.
[12] 王彩艳, 田祯祥, 刘承宜. 避孕药对不同糖质量浓度下雌性黑腹果蝇寿命的影响[J]. 华南师范大学学报: 自然科学版, 2015, 47(1):93-100.
[13] 段寅慧, 吴敏. 鬼箭羽配伍荔枝核对果蝇模型糖脂代谢的影响[J]. 吉林中医药, 2014, 34(3):278-281.
[14] 杨冬梅, 陈玲, 龙灿, 等. 北冬虫夏草提取液对果蝇繁殖力及寿命的影响[J]. 湖南农业科学, 2012 (11):87-89.
[15] 李飞, 隋新. 长白山虫草属蛹虫草多糖的抗氧化活性及其对果蝇寿命的影响[J]. 中国食品添加剂, 2015(4):69-73.
[16] 赵江, 陈纯, 刘素稳, 等. 紫薯提取物对雌性果蝇寿命的影响及其作用机制[J]. 中国食品添加剂, 2014 (7):147-152.
[17] 魏娜, 霍秀文, 张佳佳, 等. 山药粗多糖对果蝇寿命及繁殖力的影响[J]. 营养学报, 2016, 38(4):405-407.
[18] 李颖, 王瀚, 赵志强, 等. 绣球菌多糖的提取及对果蝇寿命的影响[J]. 安徽农业科学, 2014, 42(13):3863-3864.
[19] 王允, 刘冬梅, 王宇, 等. 紫甘蓝对果蝇寿命的影响及抗氧化的研究[J]. 广东化工, 2016, 43(14):10-11.
[20] 王彦平, 袁贵英, 曹娅, 等. 紫山药提取物抗氧化与延长寿命作用的研究[J]. 食品工业科技, 2017, 38(1):360-364.
[21] Ohlstein B, Spradling A.The adult Drosophila posterior midgut is maintained by pluripotent stem cells[J]. Nature, 2006, 439(7075):470-474.
[22] Micchelli CA, Perrimon N.Evidence that stem cells reside in the adult Drosophila midgut epithelium[J]. Nature, 2006, 439(7075):475-479.
[23] 楼哲丰, 曹琼洁, 冯钰淇, 等. 镉对果蝇肠道上皮细胞损伤和调控中肠干细胞增殖、分化机制的研究[J]. 中国细胞生物学学报, 2013, 35(5): 602-608.
[24] 唐润东, 毛菲菲. 食物营养对黑腹果蝇中肠干细胞的影响[J]. 昆虫学报, 2016, 59(2):148-155.
[25] Min KJ, Tatar M.Drosophila diet restriction in practice: Do flies consume fewer nutrients ?[J]. Mech Ageing Dev, 2006, 127(1):93-96.
[26] 顾蔚, 侯江瑜, 潘荣华. 高浓度酵母粉对果蝇生长及寿命的影响[J]. 中国老年学志, 2009, 29(15):1924-1926.