Effect of Runzao Mingmu Decoction on Expression of Caspase-3,Bcl-2 and Bax in Retina and Its Tissue of Rats with Acute High Intraocular Pressure

doi:10.3969/j.issn.1674-5817.2020.01.009

Abstract

Abstract: Objective To explore the possible mechanism of Runzao Mingmu Decoction in protecting optic nerve membrane cells in rats with acute high intraocular pressure. Methods Sixty male Sprague-Dawley rats were randomly divided into 6 groups. According to different treatment methods, they were divided into blank group, model group, positive drug group, Runzao Mingmu Decoction low dose group, medium dose group and high dose group. The changes of intraocular pressure and the expression of apoptosis-related factors in retinal tissue were detected and recorded in each group. Results (1) Immediately after modeling, there was no significant change in the blank group, and the intraocular pressure of the other groups was significantly increased (P<0.05). There was no significant difference in intraocular pressure between the model group, the low-dose group and the middle-dose group at 8 weeks after treatment (P>0.05), but the intraocular pressure of the high-dose group was significantly lower than that of the positive drug group (P<0.05). (2) The superoxide dismutase (SOD) level was the highest in the blank group, and the levels of malondialdehyde (MDA), nitric oxide (NO), glutamic acid (Glu) and Ca²⁺ were the lowest (P<0.05). Compared with the simple model group, there were significant differences between the drug-administered dose group and the positive drug group (P<0.05). Compared with the positive drug group, the levels of NO and Glu in the retinal tissue of the high-dose intervention group were Gradually decreasing trend, the difference was statistically significant (P<0.05). (3) The average integral optical density of Bcl-2 in rat retinal ganglion cells increased gradually with the increase of treatment dose, but there was no significant difference (P>0.05), while the average integral optical density of Bax and Caspase-3 decreased gradually, showing a dose-dependent (P<0.05). (4) Compared with the simple model group, the relative expression of Bcl-2 mRNA in the retinal ganglion cells of each group increased gradually, and the relative expression of Bax mRNA and Caspase-3 mRNA decreased gradually, all of which showed a dose-dependent difference. Statistically significant (P<0.05); the expression of Bcl-2 mRNA and Bax mRNA in the optic nerve tissue of the high dose intervention group was better than that of the positive drug group (P<0.05). Conclusions Runzao Mingmu Decoction has obvious protective effect on optic nerve in rats with acute high intraocular pressure. The mechanism may be related to the inhibition of Caspase-3/Bcl-2/Bax signaling pathway activity.

Key words: Runzao Mingmu Decoction, High intraocular pressure, Caspase-3, Bax, Bcl-2

CLC Number:

Q95-33

WAN Yu, LI Zhanmei, HUANG Hai, HUANG Xuewen, HE Yilan. Effect of Runzao Mingmu Decoction on Expression of Caspase-3,Bcl-2 and Bax in Retina and Its Tissue of Rats with Acute High Intraocular Pressure[J]. Laboratory Animal and Comparative Medicine, 2020, 40(1): 53-59.

References

[1] Dang YL, Cen YJ, Hong Y, et al.Safety and efficiency of trabectome-mediated trabecular meshwork ablation for Chinese glaucoma patients: a two-year, retrospective, multicentre study[J]. ChineseI Med J (Engl), 2018, 131(4): 420-425.
[2] Wu Y, Yu R J, Chen D, et al.Glaucoma in patients with eyes close to areas affected by port-wine stain has lateral and gender predilection[J]. Chinese Med J (Engl), 2017, 130(24):2922-2926.
[3] Liu S, Hong J, Lu X, et al.Joint Optic disc segmentation in fundus images using adversarial training[J]. Comput Biol Med, 2019, 13(2):375-381.
[4] Oltulu P, Oltulu R.The association of cataract and lens epithelial cell apoptosis in patients with pseudoexfoliation syndrome[J]. Curr Eye Res, 2017, 43(5):1-4.
[5] 曹旭, 赵静如, 许家骏, 等. 2016年美国眼科学会原发性闭角型青光眼临床指南的中西医治疗探索[J]. 中华中医药杂志, 2018, 33(8):456-459.
[6] 赵瑛, 刘立夏, 黄燕, 等. 枸杞多糖对高眼压大鼠视网膜神经节细胞的影响[J]. 中医眼耳鼻喉杂志, 2012, 2(1):24-26.
[7] 云丽娜, 崔巍. 细胞凋亡与原发性青光眼[J]. 内蒙古医学杂志, 2006, 38(3):248-250.
[8] Cordeiro MF, Normando EM, Cardoso MJ, et al.Real-time imaging of single neuronal cell apoptosis in patients with glaucoma[J]. Brain, 2017, 140(6):1757-1767.
[9] 刘蓓, 吕伯昌, 朱忠桥, 等. 葛根素对慢性高眼压模型大鼠视神经的保护作用[J]. 山东大学耳鼻喉眼学报, 2018, 32(6):104-109.
[10] Canizales L, Rodriguez L, Rivera C, et al.Low-level expression of SOD1 in peripheral blood samples of patients diagnosed with primary open-angle glaucoma[J]. Biomar Med, 2016, 10(12):1218-1223.
[11] Ferreira SM, Lerner SF, Brunzini R, et al.Oxidative stress markers in aqueous humor of glaucoma patients[J]. Am J Ophthalmol, 2004, 137(1):62-69.
[12] Rezkallah A, Kodjikian L, Malcl s A, et al. DEX implant intravitreal injection, sustained intraocular hypertension, and steroid-induced glaucoma in patients with no risk factors[J]. Graefe Arch Clin Exp, 2017, 256(1):219-220.
[13] 胡瑾, 杨静, 张开云, 等. 枸杞多糖对大鼠视神经损伤的保护作用[J]. 宁夏医科大学学报, 2017, 39(1):30-33.
[14] Alqathama A, Gibbons S, Prieto JM.Differential modulation of Bax/Bcl-2 ratio and onset of caspase-3/7 activation induced by derivatives of Justicidin B in human melanoma cells A375[J]. Oncotarget, 2017, 8(56):95999-96012.
[15] Wan L, Zhang D, Zhang J, et al.TT-1, an analog of melittin, triggers apoptosis in human thyroid cancer TT cells via regulating caspase, Bcl-2 and Bax[J]. Oncol Lett, 2017, 15(1): 1271-1278.