Abstract: Objective To observe the efiects of long-term transportation stress on autonomic nervous control of heart in two strains of Wuzhishan miniature pigs. Methods Electrocardiogram (ECG) and autonomic activities were recorded after long-lemi transportation stress for 21 h and recovered, irom conscious and unrestrained miniature pig by using a non-invasive telemetry system and the autonomic nervous ftinction was investigated by power spectral analysis of heart rate variability (ITRV). Results (I) It demonstrates an obvious circadian rhythm in Wuzhishan miniature pig, and all of results suggest that the parasympathetic nervous activity is predominant during dark phase, and sympathetic nervous activity is predominant during light phase in miniature pigs. The RR interval (RRI), the root-mean-square of successive differences (RMSSD)，short term variability (STV), very low frequency (VLF), low frequency (LF) and LF/HF in Black strain were significant lower than those of White strain (P＜0.05，P＜0.01), while heart rate (HR), long term variability (LTV), total power (TP) and high frequency (HF) were significant higher than those of white strain(P＜0.05, P＜0.01); (2) Compared with control group,RRI, SDNN, RMSSD, STV, LTV，TP，VLF, LF and LF/HF in wuzhishan miniature pig were significant decreased alter long-term transportation stress (P＜0.05，P＜0.01). while HR and HF were increased (P＜0.05，P＜0.01).The Black strain of RRI，RMSSD，STV, VLF, LF and LF/HF in the long-temi trans-portation stress group were significant lower than these of White strain (P＜0.05, P＜0.01), while HR,LTV，TP and HF were significant higher than these of White strain (P＜0.05, P＜0.01); (3) It has signifi-cant correlations between activity, heart rate and time and frequency domain indices (P＜0.05, P＜0.01).Conclusion It has significant difference on autonomic nervous control of heart between two strains of wuzhishan miniature swine induced by long-term transportation stress, and Black strain maybe easy to damage the balance. Moreover, long-term transportation stress can induce the sympathetic nervous activity decreasing，it maybe a major reason to cause of changing the biological function of miniature pig induced by long-term transportation stress.

Key words: Long-term transportation stress, Heart rate variability, Wuzhishan miniature pig;Non-invasive telemetry