Abstract: Objective To investigate and explore conditions of tansfection on the rabbit adult fibroblasts by means of electroporation, the plasmid vector of pEGFP-cl was successfully transfected into the rabbit adult fibroblasts by electroporation. Methods The rabbit fibroblasts at 7-10 passages were transferred with pEGFP-cl by means of electroporation with different electric voltages, capacitors，the doses of DNA, different incubation time before electroporation, incubation temperature after electroporation. The cell survival rate was counted under visible light microscope, and the transfection efficiency and production of protein were identified through FACS analysis. Results transfection efficiency and cell survival rate were of the higher value, when The rabbit fibroblast cell with concentra-tion of 2 × 10⁶cells/ml (electroporation medium: PBS) and mixed with 30 jig vector DNA，the electric voltage of electropration apparatus was set at 250 V, and the capacitors was 700 jiiF. Moreover，the electransfected efficiency and cell survival rate was increased with 10 min of ice incubation before electroporation and 10 min of 37℃ incubation after electroporation. Conclusion The electrotransfection is very critical for the tansfection on the rabbit adult fibroblasts，and incubation temperature could improve transfection efficiency.

Key words: Electroperation, Rabbit adult fibroblast cell, pEGFP-cl, Transfection