Abstract: Objective To investigate whether there is enrichment of NaYF₄ nano-particles in pelvic lymph node by cervical injection in rabbits, and also to decide the peak time and the best diameter of nano-particles. Methods 10% solution with different diameters of nano-particles (20、50、100 nm) is injected through female rabbit's cervix. The pelvic lymph nodes were be taken at different time (2、10、30、60、120 min) after injection and the aggregation peak of nano-particles were measured;inlO min 50 nm group, the liver、spleen and kidneys are taken with pelvic lymph nodes together to detect the aggregation characteristics of nano-particles. Result The 50 nm is a preferable NaYF₄ nano-particles diameter for NaYF₄ solution injection through cervix，and 10 min is the peak time.Compared with the liver，spleen and kidneys，more nano-particles were aggregated in pelvic lymph nodes. Conclusion NaYF₄ nanoparticle solution through cervical injection of the pelvic lymph nodes in rabbits can be enriched.

Key words: NaYF₄ Nano-particles, Rabbit model, Genital cancer, Lymph node metastasis;Lymph concentration