Abstract: Objective To observe the change of anti-oxidation activity in WHBE rabbit with irritable bowel syndrome of spleen deficiency type. Methods Twelve WHBE (white hair and black ear) rabbits and 12 Japanese white rabbits were randomly divided into two groups respectively, with 6 rabbits in normal control group and 6 rabbits in model group. The irritable bowel syndrome of spleen deficiency type rabbits model was made by using the methods of moist heat, stress and stimulation adding with taking senna decoction，and each model group rabbits were recovered naturally for 10 days after the successful establishment. The levels of superoxide dismutase (SOD), the content of malondialdehyde (MDA), the activities of glutathione peroxidase (GSH-Px), nitric oxide (NO) and nitric oxide synthase (NOS) were determined. Results Compared with normal control groups, the activities of the serum SOD and GSH-Px were significantly decreased in WHBE rabbits and Japanese white rabbits after the successful establishment of the model (P＜0.05，P＜0.01)，the content of MDA，the activities of NO and NOS were significantly increased (P＜0.05，P＜0.01). After 10 days nature recover, the activity of GSH-Px in the natural recovery groups was still lower, while the content of MDA and the activity of NOS in the WHBE rabbits were all significantly higher than those in the normal rabbits (P＜0.05). All the anti-oxidation indexes in Japanese white rabbits returned to the normal level, except for the GSH-Px activity was still higher than normal control group (P＜0.05). Conclusion The anti-oxidation activity of WHBE rabbit of irritable bowel syndrome of spleen deficiency type were significantly decreased similar to clinic in human, and recovered slowly, so the WHBE rabbits may be suitable for the study of irritable bowel syndrome of spleen deficiency type in huaman.

Key words: WHBE rabbits, Japanese white rabbits, Irritable bowel syndrome of spleen deficiency, Anti-oxidation activity