Abstract: Objective To study muscular characteristics between model mice C57BL/10ScSn-Dmd^mdx/J and background mice C57BL/6J. Methods The Diaphragmatic muscle, latissimus dorsi and gastrocnemius muscles of the CSTBL/lOScSn-Dmd^mdx/J and C57BL/6J mice were collected at the age of 2,3，and 4 weeks，respectively. Each group selected 3 mice for experiments. Dmd gene were amplified and analyzed by RT-PCR. b-dystroglycan expression was detected by Western blot. Results A mutation C — T in 3202th of Dmd gene in model mice was found, and expression of b-dystroglycan was up- regulated in C57BL/10ScSJ. Conclusion During muscle fiber development, breakage and deletion were appeared in C57BL/1 OScSn-Dmd^mdx/J and b-dystroglycan expression was up-regulated.

Key words: C57BL/10ScSn-Dmd^mdx/J model mice, C57BL/6J mice;β-dystroglycan, Western blot