›› 2008, Vol. 28 ›› Issue (2): 121-124.
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Abstract: Objective To explore the best concentration of HzOz to induce hippocampal cellular oxidative damage in rats. Method The hippocampal cell from the rat was co-cultured with H2O2 in diversity concentration for 24 hours. The changes of the cell morphology was observed, the viability of hippocampal neures was detected with MTT method; the expression of Bax and Bcl-2 were detected by immunohistochemical method. Result Compared with normal group, when the concentration of H2O2 is higher than lmmol/L,the morphology damage of hippocampal cell is obviously aggravated, the cytoactive is low,the Bax is high expression. When the concentration of H2O2 is lower than lmmol/L, the morphology damage of hippocampal cellular is not obviously aggravated, the cytoactive has no obvious change, the Bcl-2 is high expression. Conclusion The best concentration of H2O2 to establish cell oxidative damage model is lmmol/L.
Key words: Cell culture, Oxidative damage, Cell model
DIAO Bo,TANG Ying,WANG Xiao-kun,LI De-zhong. Observation on Hippocampal Cellular Oxidative Damage Induced by Different Concentration of H2O2 in Rats[J]. , 2008, 28(2): 121-124.
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https://www.slarc.org.cn/dwyx/EN/Y2008/V28/I2/121