Abstract: Objective Compare two methods for creating the rat model of acute necrotizing pancreatitis and their application. Methods Fourty SD rats were randomly divided into biliopancreatic duct directly punctured group (A group，n =16)，duodenal wall punctured group (B group, n=16) and control group (C group，n = 8). One-half of the animals of A group and B group was sacrificed 24 h after operation (as A1 group and B1 group), and the following parameters were examined: the model creating time, the volume of ascetic fluid, serum amylase and the severity of pancreatitis. Four days after operation, survival analysis of the remaining rats in A group and B group (as A2 group and B2 group) was performed. Results The histological manifestation of acute necrotizing pancreatitis was observed 24 h afler operation in A1 group and B1 group; The serum amylase and the ascetic volume of A1 group and B1 were higher obviously than C group (P<0.01), and these parameters were no different between A1 group and B1 group; The model creating time between A1 group and B1 group were different (p<0.05)，and the survival rate was significantly higher in B2 group than that in A2 group(p<0.05). Conclusion The method of puncturing duodenal wall is superior to that of directly puncturing biliopancreatic duct for creating acute necrotizing pancreatitis; while the later can be used in some short term studies because of its simple manipulation.