Laboratory Animal and Comparative Medicine ›› 2007, Vol. 27 ›› Issue (2): 99-103.

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Studies on In Vitro Fertilization by Using IVF-20 Media in Four Strains of Rat

  

  1. 1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;2. Shanghai Laboratory Animal Center, Chinese Academy of Sciences, Shanghai 201615，China;3. Comparative Medicine Center, Yangzhou University, Yangzhou 25009, China;4. College of Animal Science and Veterinary, Shenyang Agricultural University, Shenyang 110161, China
  • Received:2006-12-04 Online:2007-02-28 Published:2007-02-28

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Abstract: Objective To investigate the feasibility of IVF-20 medium on the in vitro fertilization (IVF) in Rats. Methods Using IVF-20 as capacitation and fertilization medium, the IVF in four strains, including SD, Wistar, GK and F344 were conducted. Besides, the embryos from SD rat fertilized both in vitro and in vivo were cultured using mRlEMC medium to test their viability. Result The fertilization rates in SD, Wistar, GK and F344 were 83.2%、72.6%、87.8 % and 71.6% respectively. The rate of embiyos developed to blastula was 16.7%, having comparative viability of embryos fertilized in vivo (24.5%). Conclusion The IVF-20 medium, which had been used in vitro fertilization of human, is also a suitable medium for the capacitation and fertilization in rats.

Key words: IVF-20 media, Rats, In vitro Fertilization