Abstract: Lentiviruses belong to the retroviruse and they are characterized by infecting non-dividing cells except for sharing many characteristic features with simple retroviruses. The recent studies found that the transgenic efficiency was enhanced significantly with lentiviral transgenesis. Due to its high efficiency，this method will make a breakthrough in transgenic animal research field. This article is about the principle of constructing lentiviral vector, the common approach of producing transgenic animal with lentiviral trans genes is and its latest progress home and abroad.