Abstract: Objective To investigate the levels of blood glucose, triglyceride and total cholesterol, and pathological changes of pancreas,liver and kidney in New Zealand white rabbits fed with high sucrose, high fat and high cholesterol diet. Methods Male New Zealand White rabbits were divided into two groups： control group （n= 10,with normal fodder） and high sucrose, high fat and high cholesterol diet （HD） group （n=10, with 37% sucrose, 10% lard,2% cholesterol and 51% normal fodder）, and observed for 16 weeks. Once 4 weeks after an overnight fasting, the blood samples of the rabbits were tested for the levels of serum glucose, triglyceride and total cholesterol. At the end of 16 weeks all the rabbits were sacrificed,and a part of pancreas, liver and kidney tissue were sampled for histopathological examination. Results The rabbits in the HD group emerged hyperglycemia, hypercholesterolemia and hypertriglyceridemia subsequently, and no obvious changes were observed in control group. The pancreatic islets from the HD group became atrophied, with loss in number of the islets and cells within them. The nucleus of islet cells were small and deep-dyed. There was an abundance of large vacuolations in cytoplasm. Hepatic steatosis and fibrosis, as well as glomerular hypertrophy and glomerulosclerosis, could be observed in the rabbits of HD group.It is suggested that high sugar, high fat and high cholesterol diet can increase the blood glucose, triglyceride and total cholesterol, and induce pathological changes of pancreas, liver and kidney in New Zealand White rabbits.