Abstract: To establish heart transplantation model in mini-pigs under general anesthesia and ex-tracorporeal circulation, we used eight mini-pigs to harvest donor hearts for transplantation, with general anesthesia and extracorporeal circulation, excise recipient heart, and implant donor heart in original place. The hemodynamics was monitored during the operation. After transplantation all of the donor hearts beated again.The transplantation hearts withdrawn from cardiopulmonary circulation after 60min assistant extracorporeal circulation. The hemodynamics was stable. Establishment of heart transplant model in mini-pigs was successful.