Laboratory Animal and Comparative Medicine

Identification and Analysis of the Klebsiella pneumoniae Species Complex in Laboratory Rats and Mice

QIANG Sujing, LI Dongting, XU Jinhua, CAI Li, WEI Siyu, ZOU Jialei, DING Yuqiang

Laboratory Animal and Comparative Medicine, 2026, 46(4): 564-572. DOI: 10.12300/j.issn.1674-5817.2025.174

基因 Gene	序列(5'→3'） Oligonucleotide sequences (5′→3′)	片段长度/bp Length of fragment/bp
blaLEN	F：ATTAATGTCGCTGCCCGTCA； R：CCGACGGAACTGGTAAACGA	275
blaOKP	F：GATCGCCCTGATCGGCTGCT； R：CCGAACGCCAGCGACCACTG	372
blaSHV	F：TGCTTGCCAATGTCTGGGAT； R：TCTGGCCTGCGTCGCAGCAT	650

基因

Gene

序列(5'→3'）

Oligonucleotide sequences (5′→3′)

片段长度/bp

Length of fragment/bp

blaLEN

F：ATTAATGTCGCTGCCCGTCA；

R：CCGACGGAACTGGTAAACGA

275

blaOKP

F：GATCGCCCTGATCGGCTGCT；

R：CCGAACGCCAGCGACCACTG

372

blaSHV

F：TGCTTGCCAATGTCTGGGAT；

R：TCTGGCCTGCGTCGCAGCAT

650

Table 1 Primer sequences for multiplex PCR

Other figure/table from this article

Table 2 Primer sequences for the detection of virulence genes in Klebsiella pneumoniae
Figure 1 Multiplex PCR results （A） and culture results（B） of KP1-KP3
Note： M， DNA marker； lanes 1-4， approximately 372 bp （Kp2）； lanes 5-7 and 9， approximately 650 bp （Kp1）； lanes 8 and 10-11， approximately 275 bp （Kp3）； NC， negative control. a and d， Kp1； b and e， Kp2； c and f， Kp3.
Table 3 Identification results of isolated strains
Table 4 Detection of virulence genes in Kp1-Kp3
Table 5 Results of antimicrobial susceptibility testing of Kp1-Kp3