Abstract: Objective To investigate the enhanced immunity of hepatitis B surface antigen (HBsAg) vaccine in response to interleukin-12 (IL-12) stimulation. Method C57BL/6J mice were injected with rmIL-12 plus HBsAg combination，after which the serum IFN-T level as well as the response of murine spleen lymphocytes to HBsAg was detected. Results Serum IFN-T level in high dose combination of HBsAg and rmIL-12 group after two injections was significant higher than that in HBsAg or rmIL-12 alone (尸<0.05). The ability of murine spleen lymphocytes producing IFN-T in response to HBsAg was significantly improved (P<0.05), and IFN-T mainly originated from CD4+ & CD8+ T cells detected by flow cytometric analysis. Conclusion The immunity response of C57BL/6J mice to HBsAg can be rapidly and specifically enhanced by rmIL-12, which may be a reference of IL-12 plus HBsAg clinical application on CHB infection.

Key words: CHB, rmIL-12, HBsAg, IFN-T