自主神经调节剂对房颤模型大鼠心房电重塑及组织病理变化的影响

李嘉菲, 张朕豪, 王硕, 田歌, 温爽, 闫玉雪, 崔然, 叶振, 崔永春

Effects of Autonomic Neuromodulators on Atrial Electrical Remodeling and Histopathological Changes in a Rat Model of Atrial Fibrillation

LI Jiafei, ZHANG Zhenhao, WANG Shuo, TIAN Ge, WEN Shuang, YAN Yuxue, CUI Ran, YE Zhen, CUI Yongchun

图 3 自主神经调节剂诱发大鼠心房肌细胞形态和氧化应激水平变化 注：A，心房肌细胞WGA染色代表性图片；B，心房肌组织DHE染色代表性图片及TUNEL染色代表性图片；C，心肌细胞横截面积定量分析；D，心房肌组织DHE染色的荧光强度定量分析；E，细胞凋亡率对比分析。WGA，麦胚凝集素；α-Actinin，α辅肌动蛋白；DHE，二氢乙锭；TUNEL，末端脱氧核苷酸转移酶介导的dUTP缺口末端标记；DAPI，4'，6-二脒基-2-苯基吲哚。每组2只大鼠共3张切片进行统计学分析（n=3）；与对照组相比，*P<0.05，**P<0.01，***P<0.001。

Figure 3 Autonomic neuromodulators induce morphological and oxidative stress changes in rat atrial cardiomyocytes Note： A， Representative images of WGA staining of atrial cardiomyocytes； B， Representative images of DHE staining and TUNEL staining of atrial myocardial tissues； C， Quantitative analysis of cardiomyocyte cross-sectional area； D， Quantitative analysis of the fluorescence intensity of DHE staining in myocardial tissues； E， Comparative analysis of apoptosis rate. WGA， wheat-germ agglutinin； DHE， dihydroethidium； TUNEL， terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. DAPI， 4'，6-diamidino-2-phenylindole.Three sections from two rats per group were subjected to statistical analysis （n=3). *P < 0.05， **P < 0.01， ***P < 0.001， vs the control group.