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基于miRNA测序分析清肺排毒汤防治小鼠急性肺损伤的分子机制研究
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李龙雪 1(  ), 万崇凡 2, 张琦 2, 雷茹婷 2, 王潇玥 3, 程乐妍 2, 赖琦 2, 刘荣华 4( )(  ), 刘漩 1( )( ), 徐铁龙 5( )( )
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Molecular Mechanisms of Qingfei Paidu Decoction in the Prevention and Treatment of Acute Lung Injury in Mice Based on miRNA Sequencing
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LI Longxue 1( ), WAN Chongfan 2, ZHANG Qi 2, LEI Ruting 2, WANG Xiaoyue 3, CHENG Leyan 2, LAI Qi 2, LIU Ronghua 4( )( ), LIU Xuan 1( )( ), XU Tielong 5( )( )
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图2. miRNA测序分析各组小鼠肺组织中差异表达的miRNA
注：A，韦恩图，橙色圆圈代表QFPDD组与模型组相比具有显著性变化的miRNA个数，蓝色圆圈代表模型组与对照组相比具有显著性变化的miRNA个数，重叠部分代表两次比较中共有的miRNA个数。B，与QFPDD的潜在防治效应相关的miRNA表达量变化。对照组，连续8 d灌胃给予超纯水；模型组，灌胃给予超纯水1 d，然后于第2～8天给予脂多糖（LPS）水溶液雾化以建立急性肺损伤模型；QFPDD组，连续8 d灌胃给予清肺排毒汤（QFPDD），并于第2～8天给予LPS水溶液雾化建模。每组随机选取4只小鼠的肺组织，与对照组比较，*P＜0.05，**P＜0.01；与模型组比较，#P＜0.05，##P＜0.01。
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Figure 2. Differentially expressed miRNAs in lung tissues of mice among groups detected by miRNA sequencing
Note： A， Venn diagram. The orange circle represents the number of miRNAs with significant changes in the QFPDD group compared with the model group. The blue circle represents the number of miRNAs with significant changes in the model group compared with the control group. The overlapping section represents the number of miRNAs shared in both comparisons. B， Changes in the expression levels of miRNAs associated with the potential preventive and therapeutic effects of QFPDD. Control group， intragastrically administered ultrapure water for 8 consecutive days. Model group， intragastrically administered ultrapure water on day 1， followed by exposure to aerosolized lipopolysaccharide （LPS） solution on days 2–8 to establish the acute lung injury （ALI） model. QFPDD group， intragastrically administered Qingfei Paidu decoction （QFPDD） for 8 consecutive days， with concurrent exposure to aerosolized LPS solution on days 2–8 for ALI induction. Lung tissues from 4 randomly selected mice per group were used （n=4）. Compared with the control group， *P < 0.05， **P < 0.01； compared with the model group， #P < 0.05， ##P < 0.01.
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