脊髓压迫和半横断损伤小鼠模型的时空基因表达谱及修复机制研究

徐波, 陈泰任, 方乾, 吴际

Research on Spatiotemporal Gene Expression Profiles and Repair Mechanisms of Spinal Cord Compression and Hemisection Spinal Cord Injury Mouse Models

XU Bo, CHEN Tairen, FANG Qian, WU Ji

图4 实时荧光定量PCR验证表达差异基因的mRNA相对表达水平 注：A~D，与CTR组相比，SCC组在第7和21天上调和下调关键基因的实时荧光定量PCR结果；E~H，与CTR组相比，HSCI组在第7和21天上调和下调关键基因的实时荧光定量PCR结果。CTR，假手术对照组；SCC，脊髓压迫损伤组；HSCI，脊髓半横断损伤组。图中数据均以CTR组基因表达水平为1进行归一化展示。实时荧光定量PCR实验使用RNA测序后剩余的同一批小鼠组织样本进行检测（n=3）。?P<0.05，??P<0.01，???P<0.001。

Figure 4 Validation of mRNA expression levels of differentially expressed genes by real time fluorogenic quantitative PCR Note： A-D， real time fluorogenic quantitative PCR results of the key genes that up-regulated and down-regulated in the SCC group compared with the CTR group at days 7 and 21； E-H， real time fluorogenic quantitative PCR results of the key genes that up-regulated and down-regulated at days 7 and 21 in the HSCI group compared with the CTR group. CTR， sham-operated control group； SCC， spinal cord contusion injury group； HSCI， hemisection spinal cord injury group. All data in the figure are normalized to a baseline of 1 for the CTR group. Real time fluorogenic quantitative PCR experiments were performed using the same batch of mouse tissue samples remaining after RNA-seq（n=3）. ?P<0.05， ??P<0.01， ???P<0.001.