外泌体通过NRF2/SLC7A11/GPX4通路调控铁死亡治疗小鼠缺血性脑卒中

徐英韬, 王蒙蒙, 林平, 迟海涛, 王怡, 白鹰

Exosomes Treat Ischemic Stroke by Regulation of Ferroptosis Through the NRF2/SLC7A11/GPX4 Pathway in Mice

XU Yingtao, WANG Mengmeng, LIN Ping, CHI Haitao, WANG Yi, BAI Ying

图5 各组小鼠脑组织中Fe2+、MDA、total GSH、GSSG和GSH含量的微量比色法分析结果 注：A~E，各组小鼠脑组织中亚铁离子（Fe2+）、丙二醛（MDA）、总谷胱甘肽（total GSH）、氧化型谷胱甘肽（GSSG）和还原型谷胱甘肽（GSH，即total GSH和GSSG含量的差值）含量分析。假手术组（Sham）只暴露大脑中动脉，不电凝血管；模型组（MCAO）使用高频手术镊以8 W功率电凝大脑中动脉；模型+生理盐水组（MCAO+NaCl）先通过尾静脉注射100 μL生理盐水，然后进行电凝法造模；模型+外泌体组（MCAO+EXO）先通过尾静脉注射100 μL来自人羊膜间充质干细胞（hAMSCs）培养上清液的外泌体（9.5×1011个/mL），然后进行电凝法造模。每组小鼠各8只，nsP >0.05，??P＜0.01，???P＜0.001，????P＜0.000 1。

Figure 5 Microcolorimetric assays of Fe2+， MDA， total GSH， GSSG and GSH contents in the brains of each group of mice Note： A-E， Contents of Fe2+， malondialdehyde （MDA）， total glutathione （total GSH）， oxidized glutathione （GSSG）， and reduced glutathione （GSH） in the brains of mice from each group. Reduced GSH was calculated as the difference between the contents of total GSH and GSSG. The sham operation group （Sham） only exposed the middle cerebral artery without electrocoagulation of the vessel； the model group （MCAO） used high-frequency surgical forceps to electrocoagulate the middle cerebral artery at a power of 8 W； the model + normal saline group （MCAO+NaCl） first injected 100 μL of normal saline through the tail vein， and then performed electrocoagulation modeling； the model + exosome group （MCAO+EXO） first injected 100 μL of exosomes （9.5×1011 particles per mL） from the culture supernatant of human amniotic mesenchymal stem cells （hAMSCs） through the tail vein， and then performed electrocoagulation modeling. There were eight mice in each group. nsP >0.05， ??P<0.01， ???P<0.001， ????P<0.000 1.