外泌体通过NRF2/SLC7A11/GPX4通路调控铁死亡治疗小鼠缺血性脑卒中

徐英韬, 王蒙蒙, 林平, 迟海涛, 王怡, 白鹰

Exosomes Treat Ischemic Stroke by Regulation of Ferroptosis Through the NRF2/SLC7A11/GPX4 Pathway in Mice

XU Yingtao, WANG Mengmeng, LIN Ping, CHI Haitao, WANG Yi, BAI Ying

图3 各组小鼠神经功能缺损评分及脑梗死体积占比分析 注：A，各组小鼠神经功能Longa评分；B，各组小鼠脑切片的2，3，5-三苯基氯化四氮唑（TTC）染色结果，每列6张切片为1只小鼠的完整大脑，正常脑组织为红色，梗死区域脑组织为白色；C，各组小鼠脑梗死体积占比。假手术组（Sham）只暴露大脑中动脉，不电凝血管；模型组（MCAO）使用高频手术镊以8 W功率电凝大脑中动脉；模型+生理盐水组（MCAO+NaCl）先通过尾静脉注射100 μL生理盐水，然后进行电凝法造模；模型+外泌体组（MCAO+EXO）先通过尾静脉注射100 μL来自人羊膜间充质干细胞（hAMSCs）培养上清液的外泌体（9.5×1011个/mL），然后进行电凝法造模。每组小鼠各8只，??P＜0.01，???P＜0.001。

Figure 3 Analysis of neurological deficit scores and the percentages of cerebral infarction volume in each group of mice Note： A， Neurological Longa scores of each group of mice； B， 2，3，5-triphenyltetrazolium chloride （TTC） staining results of brain sections in each group of mice， each column contains six slices representing the complete brain of one mouse， normal brain tissue was red， while brain tissue in the infarcted area was white； C， The percentages of brain infarction volume in each group of mice. The sham operation group （Sham） only exposed the middle cerebral artery without electrocoagulation of the vessel； the model group （MCAO） used high-frequency surgical forceps to electrocoagulate the middle cerebral artery at a power of 8 W； the model + normal saline group （MCAO+NaCl） first injected 100 μL of normal saline through the tail vein， and then performed electrocoagulation modeling； the model + exosome group （MCAO+EXO） first injected 100 μL of exosomes （9.5×1011 particles per mL） from the culture supernatant of human amniotic mesenchymal stem cells （hAMSCs） through the tail vein， and then performed electrocoagulation modeling. There were eight mice in each group. ??P＜0.01， ???P＜0.001.