树鼩Vasorin单克隆抗体的制备及其应用探索

欧美珍, 李泳锋, 温莎, 廖舟翔, 黄雪静, 何敏, 杨丽超

Preparation of Monoclonal Antibody to Vasorin in Tree Shrew and Exploration of Its Application

OU Meizhen, LI Yongfeng, WEN Sha, LIAO Zhouxiang, HUANG Xuejing, HE Min, YANG Lichao

图4 树鼩VASN抗体的应用 注：A，蛋白质印迹法鉴定树鼩各组织中VASN的蛋白表达水平（M，蛋白质分子量标准；1，心脏组织；2，肝脏组织；3，脾脏组织；4，肺脏组织；5，肾脏组织；6，肌肉组织）；B，实时荧光定量PCR检测树鼩各组织中VASN mRNA的相对表达水平（与肺脏组织比较，***P<0.001，****P<0.000 1；与脾脏组织比较，###P<0.001，####P<0.000 1）；C，VASN基因扩增结果（M，DNA分子量标准；1，阳性对照；2，VASN扩增产物；3，空白对照）；D，抗体应用于免疫组织化学鉴定树鼩永生化成纤维细胞中VASN蛋白的表达；E，抗体应用于免疫组织化学鉴定脾脏组织中VASN蛋白的表达；F，抗体应用于免疫组织化学鉴定肺脏组织中VASN蛋白的表达。

Figure 4 Application of tree shrews VASN antibody Note：A， The protein expression level of VASN in various tissues of tree shrew was identified by Western blotting（M， Protein marker； 1， Heart tissue； 2， Liver tissue； 3， Spleen tissue； 4， Lung tissue； 5， Kidney tissue； 6， Muscle tissue）； B， Relative expression levels of VASN mRNA in various tissues of tree shrews detected by qRT-PCR （Compared with lung tissue，***P<0.001，****P<0.000 1； compared with spleen tissue， ###P<0.001，####P<0.000 1）； C， Amplification results of the VASN gene（M， DNA Marker； 1， Positive control； 2， VASN amplification product； 3， Blank control）； D， Antibodies were used in immunohistochemistry to identify the expression of VASN protein in immortalized fibroblasts of tree shrews； E， Antibodies were used in immunohistochemistry to identify the expression of VASN protein in spleen tissue； F， Antibodies were used in immunohistochemistry to identify the expression of VASN protein in lung tissue.