树鼩Vasorin单克隆抗体的制备及其应用探索
欧美珍, 李泳锋, 温莎, 廖舟翔, 黄雪静, 何敏, 杨丽超

Preparation of Monoclonal Antibody to Vasorin in Tree Shrew and Exploration of Its Application
OU Meizhen, LI Yongfeng, WEN Sha, LIAO Zhouxiang, HUANG Xuejing, HE Min, YANG Lichao
图2 VASN重组蛋白的表达与纯化
注:A,VASN重组蛋白的表达(M,蛋白质分子量标准;1,未诱导的pET-30a-VASN上清;2,未诱导的pET-30a-VASN沉淀;3,经诱导的pET-30a-VASN上清;4,经诱导的pET-30a-VASN沉淀);B,VASN重组蛋白的纯化(M,蛋白质分子量标准;1,VASN重组蛋白纯化前;2,VASN重组蛋白纯化后;3,蛋白上样缓冲液)。
Figure 2 Expression and purification of VASN recombinant protein
Note: A, Expression of VASN recombinant protein (M, Protein marker; 1, Uninduced pET-30a-VASN supernatant; 2, Uninduced pET-30a-VASN precipitate; 3, Induced pET-30a-VASN superna-tant; 4, Induced pET-30a-VASN precipitate); B, Purification of VASN recombinant protein (M, Protein marker; 1, Before purifi-cation of VASN recombinant protein; 2, After purification of VASN recombinant protein; 3, Protein loading buffer).