中医证候类动物模型构建方法概述
|
|
王笑铭, 孟晨晨, 范鹿, 李艳阳, 张军平, 吕仕超
|
An Overview of Strategies for Constructing Animal Models of Traditional Chinese Medicine Syndromes
|
|
WANG Xiaoming, MENG Chenchen, FAN Lu, LI Yanyang, ZHANG Junping, LÜ Shichao
|
|
表3 常见的中西医病证结合构建方法、实验动物选择、作用机制以及中医证型
|
Table 3 Common construction methods, laboratory animal selections, mechanisms, and traditional Chinese medicine syndromes of integration of traditional Chinese medicine and Western medicine for diseases and syndromes
|
|
造模方法 Construction methods | 实验动物选择 Laboratory animal selections | 作用机制 Mechanisms | 中医证型 Traditional Chinese medicine syndromes |
|---|
异硫氰酸萘酯灌胃法结合湿热环境法、高糖高脂饮食法[53] Oral administration of α-naphthyl isothiocyanate along with the hot and humid environment and high-fat and high-carbohydrate diet[53] | 雄性SD大鼠 | 异硫氰酸萘酯灌胃强化肝损伤，胆红素代谢障碍，导致黄疸；外感湿热之邪、高糖高脂的饮食因素诱发脾胃气机失调、湿热内蕴，引起阳黄证 | 黄疸阳黄证 | 冠状动脉结扎法联合高脂饮食法[55] Coronary artery ligation combined with a high-fat feeding diet[55] | 雄性Wistar大鼠 | 冠状动脉结扎直接阻断血流，血行不畅，瘀阻脉络，符合“瘀证”；高脂饮食导致痰浊内生，壅塞脉道，阻滞气机，形成“痰证”；二者结合，形成痰瘀互结证 | 冠心病痰瘀互结证 | 灌服盐酸普萘洛尔法联合负重力竭游泳运动法[56] Oral administration of propranolol hydrochloride solution and weight-loaded exhaustive swimming[56] | 雄性KM小鼠 | 盐酸普萘洛尔减慢心率、抑制心脏收缩力；过劳伤脾，脾胃虚弱，气血生化无源；二者结合，导致心神失养，发为心脾两虚证失眠 | 失眠心脾两虚证 | 病毒滴鼻感染法联合湿热环境法[59] External damp-heat stimulation and viral intranasal infection[59] | BALB/c小鼠，雌雄各半 | 滴鼻感染直接侵袭呼吸道黏膜上皮细胞；疫毒外侵，正气亏虚，肺脏受邪；湿性黏滞、热性炎上，湿热环境导致气机壅滞，津液输布失常，湿热内蕴，熏蒸肺卫，发为湿热疫 | 人冠状病毒肺炎湿热疫毒袭肺证 | 皮内注射Ⅱ型胶原法结合寒湿环境法（或湿热环境法）[61] Intradermal injection of type Ⅱ collagen combined with the cold and damp environment (or hot and humid environment)[61] | Wistar大鼠 | Ⅱ型胶血管翳形成及软骨破坏，寒湿侵袭关节，寒性收引、湿性黏滞，导致经络闭阻、气血运行不畅，形成寒湿痹证 | 类风湿关节炎寒湿证 | | Ⅱ型胶血管翳形成及软骨破坏，湿热内蕴，熏蒸关节，炼液成痰、灼血成瘀，瘀则不通，不通则痛，发为湿热痹证 | 类风湿关节炎湿热证 |
|
|
|