药物非临床生殖毒性试验中动物妊娠的主要难点及应对方法

刘鹍, 兰青, 易兵, 谢晓婕

Key Challenges and Mitigation Strategies for Animal Pregnancy in Non-clinical Reproductive Toxicity Testing of Drugs

LIU Kun, LAN Qing, YI Bing, XIE Xiaojie

图1 成年雌性大鼠动情周期各阶段的典型阴道组织涂片 注：A，动情前期；B，动情期；C，动情后期；D，间情期。黑色箭头所指的是有核上皮细胞；红色箭头所指的是无核上皮细胞；绿色箭头所指的是白细胞。涂片的染色方法为HE染色。图像的放大倍数为40，标尺为100 μm。

Figure 1 Typical vaginal smear characteristics from adult female rats at each stage of estrous cycle Note：A， proestrus； B， estrus； C， metestrus； D， diestrus. The black arrow indicates nucleated epithelial cells； the red arrow indicates anucleated epithelial cells； the green arrows indicate leukocytes. The staining method of the smear was hematoxylin and eosin （HE） staining. The magnification of the images is 40 and the scale bars are 100 μm.