慢性肾脏病大鼠主动脉钙化模型的术式优化及效果评价

潘颐聪, 蒋汶洪, 胡明, 覃晓

Optimization of Surgical Procedure and Efficacy Evaluation of Aortic Calcification Model in Rats with Chronic Kidney Disease

PAN Yicong, JIANG Wenhong, HU Ming, QIN Xiao

表1 PCR引物序列信息

Table 1 Primers for real-time fluorescent quantitative PCR

目标基因名称 Target gene name	NCBI基因序列号 NCBI reference sequence	引物序列信息 Primer sequence information	扩增片段大小/bp Amplification fragment size/bp
Sm22 (Tagln)	NC-086026.1	F：5’-CAGATGGAACAGGTGGCTCAA-3’	161
		R：5’-GCCCAAAGCCATTACAGTCCTC-3’
OPN (Spp1)	NC-086032.1	F：5’-GCCGAGGTGATAGCTTGGCTTA-3’	145
		R：5’-TTGATAGCCTCATCGGACTCCTG-3’
Runx2	NC-086027.1	F：5’-GGATGCCTTAGTGCCCAAATG-3’	120
		R：5’-CACCCTGTGAGGTGGCTGAA-3’
β-actin	NC-086030.1	F：5’-CACCCGCGAGTACAACCTTC-3’	207
		R：5’-CCCATACCCACCATCACACC-3’