慢性肾脏病大鼠主动脉钙化模型的术式优化及效果评价

潘颐聪, 蒋汶洪, 胡明, 覃晓

Optimization of Surgical Procedure and Efficacy Evaluation of Aortic Calcification Model in Rats with Chronic Kidney Disease

PAN Yicong, JIANG Wenhong, HU Ming, QIN Xiao

图5 实时荧光定量PCR检测实验组和对照组大鼠的主动脉组织细胞中OPN、Runx2及Sm22基因表达 注：相较于对照组（sham），实验组（CI）中反映血管平滑肌成骨样分化表型的OPN及Runx2相对表达量明显增加（*P<0.05，**P<0.01），而反映血管平滑肌收缩表型的Sm22表达量减少（***P<0.001）。

Figure 5 Real-time fluorescence quantitative PCR detection of OPN， Runx2， and Sm22 gene expression in aortic tissue of rats from the experimental and control groups Note： Compared with the control group （sham）， the expression level of Sm22， which reflects the vascular smooth muscle contraction phenotype， decreased in the experimental group （CI） （***P<0.001）， while the expression levels of OPN and Runx2， which reflects the osteogenic differentiation phenotype of vascular smooth muscle， increased significantly （ * P<0.05， ** P<0.01）.