利用脑立体定位技术将人源三突变APP基因导入海马区构建阿尔茨海默病大鼠模型

肖林林, 杨逸萱, 黎珊杉, 罗兰诗雨, 尹思威, 孙俊铭, 施维, 欧阳轶强, 李习艺

Establishment of a Rat Model of Alzheimer's Disease by Introducing Human Triple Mutant APP Gene into Hippocampus via Brain Stereotactic Technology

XIAO Linlin, YANG Yixuan, LI Shanshan, LUO Lanshiyu, YIN Siwei, SUN Juming, SHI Wei, OUYANG Yiqiang, LI Xiyi

图5 大鼠海马体组织的HE染色（A）、尼氏染色（B）和免疫组织化学染色（C）结果 注：BC、VC、Exp分别为空白对照组、空载病毒组、实验组［通过脑立体定位在海马区注射携带人源三突变淀粉样前体蛋白（APP）和NanoLuc萤光素酶基因的腺相关病毒］。黑色箭头所指区域为皱缩坏死的细胞；红色箭头所指区域为尼氏体稀疏，染色浅淡的细胞；蓝色箭头所指区域为β-淀粉样蛋白沉积区域。

Figure 5 HE staining （A）， Nissl staining （B）， and immunohistochemical staining （C） results of rat hippocampal region Note： BC， VC， Exp are blank control group， virus control group and experimental group （adeno-associated virus carrying human triple mutant APP and NanoLuc luciferase genes was injected in the hippocampus by brain stereotaxic localization）， respectively. The areas indicated by black arrows are crumpled necrotic cells. The areas indicated by red arrows are cells with sparse Nissl bodies and light staining. The area indicated by blue arrow is the area of amyloid β-protein deposition.