利用脑立体定位技术将人源三突变APP基因导入海马区构建阿尔茨海默病大鼠模型

肖林林, 杨逸萱, 黎珊杉, 罗兰诗雨, 尹思威, 孙俊铭, 施维, 欧阳轶强, 李习艺

Establishment of a Rat Model of Alzheimer's Disease by Introducing Human Triple Mutant APP Gene into Hippocampus via Brain Stereotactic Technology

XIAO Linlin, YANG Yixuan, LI Shanshan, LUO Lanshiyu, YIN Siwei, SUN Juming, SHI Wei, OUYANG Yiqiang, LI Xiyi

图4 大鼠海马体APP 基因表达及测序结果 注：A，为实时荧光定量PCR检测结果［BC、VC、Exp分别为空白对照组、空载病毒组、实验组（通过脑立体定位在海马区注射携带人源三突变APP和NanoLuc萤光素酶基因的腺相关病毒），每组4只大鼠（n=4）；与空白对照组相比，**P<0.01；与空载病毒组相比，##P<0.01］。B为PCR产物测序比对结果，黄色高亮部分指示突变位点。

Figure 4 APP gene expression and sequencing results in rat hippocampus Note： A is the result of real-time fluorescence quantitative PCR. BC， VC， Exp are blank control group， virus control group and experimental group （adeno-associated virus carrying human triple mutant APP and NanoLuc luciferase genes was injected in the hippocampus by brain stereotaxic localization）， respectively. 4 rats in each group （n=4）； Compared with blank control group， **P<0.01； Compared with virus control group， ##P<0.01. B is the result of real-time fluorescence quantitative PCR product sequencing comparison， and the yellow highlighted part is the indicated mutation site.