利用脑立体定位技术将人源三突变APP基因导入海马区构建阿尔茨海默病大鼠模型

肖林林, 杨逸萱, 黎珊杉, 罗兰诗雨, 尹思威, 孙俊铭, 施维, 欧阳轶强, 李习艺

Establishment of a Rat Model of Alzheimer's Disease by Introducing Human Triple Mutant APP Gene into Hippocampus via Brain Stereotactic Technology

XIAO Linlin, YANG Yixuan, LI Shanshan, LUO Lanshiyu, YIN Siwei, SUN Juming, SHI Wei, OUYANG Yiqiang, LI Xiyi

图3 新物体识别实验检测大鼠认知记忆能力 注：A展示大鼠探索两个相同物体；B为更换了新物体；C为以距离为统计指标的新物体识别实验结果；D为以时间为统计指标的新物体识别实验结果。RI：新事物识别指数。BC、VC、Exp分别为空白对照组、空载病毒组、实验组（通过脑立体定位在海马区注射携带人源三突变APP和NanoLuc萤光素酶基因的腺相关病毒），每组8只大鼠（n=8）；与空白对照组相比，**P<0.01；与空载病毒组相比，#P<0.05。

Figure 3 Novel object recognition test to assess recognition memory of rats Note：A shows rats exploring two identical objects； B is a new object replaced； C is the result of the new object recognition experiment using distance as a statistical index； D is the result of the new object recognition experiment using time as a statistical index. RI：Recognition index.BC， VC， Exp are blank control group， virus control group and experimental group （adeno-associated virus carrying human triple mutant APP and NanoLuc luciferase genes was injected in the hippocampus by brain stereotaxic localization）， respectively， 8 rats per group （n=8）； **P<0.01， compared with blank control group； #P<0.05， compared with virus control group.