利用脑立体定位技术将人源三突变APP基因导入海马区构建阿尔茨海默病大鼠模型

肖林林, 杨逸萱, 黎珊杉, 罗兰诗雨, 尹思威, 孙俊铭, 施维, 欧阳轶强, 李习艺

Establishment of a Rat Model of Alzheimer's Disease by Introducing Human Triple Mutant APP Gene into Hippocampus via Brain Stereotactic Technology

XIAO Linlin, YANG Yixuan, LI Shanshan, LUO Lanshiyu, YIN Siwei, SUN Juming, SHI Wei, OUYANG Yiqiang, LI Xiyi

图1 重组腺相关病毒载体结构示意图 注：A为空载病毒质粒结构图；B为携带人源三突变APP基因的质粒结构图。APPsla-L-A代表人源三突变APP基因，Nluc代表NanoLuc萤光素酶基因，SYN promoter是神经元特异性启动子。

Figure 1 Schematic diagram of recombinant adeno-associated viral vector structure Note：A， structure of the empty viral plasmid； B， structure of the plasmid carrying the human triple mutant APP gene， APPsla-L-A represents the human triple mutant APP gene， Nluc represents the NanoLuc luciferase gene， and SYN promoter is the neuron-specific promoter.