秦皮素对碘乙酸钠诱导骨关节炎模型大鼠的软骨保护与抗炎作用

刘智伟, 杨然, 连浩, 张玉, 金立伦

Cartilage Protection and Anti-Inflammatory Effects of Fraxetin on Monosodium Iodoacetate-Induced Rat Model of Osteoarthritis

LIU Zhiwei, YANG Ran, LIAN Hao, ZHANG Yu, JIN Lilun

图2 碘乙酸钠注射造模及秦皮素药物干预后大鼠膝关节的micro-CT成像 注：A、B、C、D分别从膝关节3D层面、胫骨平台、软骨下骨水平面以及横截面特定区域截面扫描3组大鼠膝关节，观察相关骨质情况，评价骨关节炎情况。空白组指先向右膝关节腔注射50 μL生理盐水，再腔内连续7 d注射50 μL生理盐水的大鼠；模型组指右膝关节腔内单次注射50 μL新鲜配制的60 mg/mL碘乙酸钠建立膝骨关节炎动物模型，再连续7 d注射50 μL生理盐水的大鼠；干预组指右膝关节腔内单次注射50 μL新鲜配制的60 mg/mL碘乙酸钠后，再连续7 d每天注射5 mg/kg秦皮素（溶于50 μL生理盐水）的大鼠。

Figure 2 Micro-CT imaging of the rat knee joint after monosodium iodoacetate injection and fraxetin treatment Note： A， B， C， and D represent micro-CT images of the knee joints of three groups of rats， scanned at the levels of 3D reconstruction， the tibial platform， the subchondral bone， and specific cross-sectional regions， to assess bone structure and evaluate osteoarthritis changes. The blank group refers to rats that were first injected with 50 μL of normal saline into the right knee joint cavity， followed by intra-articular injections of 50 μL of normal saline for 7 consecutive days； the model group refers to rats that were injected with one time injection of 50 μL fresh prepared monosodium iodoacetate （60 mg/mL）in the right knee joint cavity to induce osteoarthritis， followed by injection of 50 μL of normal saline for 7 consecutive days； the intervention group refers to rats that were injected with one time injection of 50 μL fresh prepared monosodium iodoacetate （60 mg/mL）in the right knee joint cavity， followed by intra-articular injections of fraxetin （5 mg·kg-1·d-1， dissolved in 50 μL of normal saline） for 7 consecutive days.