秦皮素对碘乙酸钠诱导骨关节炎模型大鼠的软骨保护与抗炎作用

刘智伟, 杨然, 连浩, 张玉, 金立伦

Cartilage Protection and Anti-Inflammatory Effects of Fraxetin on Monosodium Iodoacetate-Induced Rat Model of Osteoarthritis

LIU Zhiwei, YANG Ran, LIAN Hao, ZHANG Yu, JIN Lilun

图1 碘乙酸钠注射造模及秦皮素药物干预后大鼠膝关节软骨组织病理学变化 注：A、B、C分别为3组大鼠膝关节软骨组织的苏木精-伊红、番红O-固绿、甲苯胺蓝染色结果（比例尺为300 μm，黑色箭头指向膝关节软骨表面）。空白组指先向右膝关节腔注射50 μL生理盐水，再腔内连续7 d注射50 μL 生理盐水的大鼠；模型组指右膝关节腔内单次注射50 μL新鲜配制的（60 mg/mL）碘乙酸钠建立膝骨关节炎动物模型，再连续7 d注射50 μL 生理盐水的大鼠；干预组指右膝关节腔内单次注射50 μL新鲜配制的（60 mg/mL）碘乙酸钠后，再连续7 d每天注射5 mg/kg秦皮素（溶于50 μL 生理盐水）的大鼠。

Figure 1 Histopathological changes in rat knee joint cartilage after monosodium iodoacetate injection and fraxetin intervention Note： A， B， and C are the staining results of hematoxylin-eosin， safranin O–fast green， and toluidine blue in the knee cartilage tissue of three groups of rats （scale bar = 300 μm， the black arrow in the picture points to the surface of the knee cartilage）. The blank group refers to rats that were first injected with 50 μL of normal saline into the right knee joint cavity， followed by intra-articular injections of 50 μL of normal saline for 7 consecutive days； the model group refers to rats that were injected with one time injection of 50 μL fresh prepared monosodium iodoacetate （60 mg/mL）in the right knee joint cavity to induce osteoarthritis， followed by injections of 50 μL of normal saline for 7 consecutive days； the intervention group refers to rats that were injected with one time injection of 50 μL fresh prepared monosodium iodoacetate （60 mg/mL）in the right knee joint cavity， followed by fraxetin injections （5 mg·kg-1·d-1， dissolved in 50 μL of normal saline） for 7 consecutive days.