来曲唑缓释片皮下给药构建小鼠多囊卵巢综合征模型及其肝脏转录组学分析

许秋雨, 严国锋, 付丽, 范文华, 周晶, 朱莲, 仇淑雯, 张洁, 吴铃

A Mouse Model of Polycystic Ovary Syndrome Established Through Subcutaneous Administration of Letrozole Sustained-Release Pellets and Hepatic Transcriptome Analysis

XU Qiuyu, YAN Guofeng, FU Li, FAN Wenhua, ZHOU Jing, ZHU Lian, QIU Shuwen, ZHANG Jie, WU Ling

图 1 来曲唑和高脂饮食造模对小鼠体重、摄食量和肝脏的影响 注：A，与初始体重相比，小鼠前4 周每周体重变化率；B，小鼠造模期间每周24 h摄食量；C，小鼠卵巢HE染色（×50）；D，小鼠肝脏重量；E，小鼠肝重比；F，小鼠肝脏HE染色（×400）；G，小鼠肝脏油红O染色（×400）。每组8只小鼠；与安慰剂组相比，*P<0.05，***P<0.001，nsP>0.05。C、F和G图中，上图与下图分别代表两个独立的生物学重复实验。

Figure 1 Effects of letrozole and high-fat diet modeling on body weight， food intake and liver in mice Note： A， Weekly rate of body weight change of mice during the first 4 weeks compared to their initial body weight； B， Weekly food intake of mice in 24 hours during the modeling period； C， Ovary HE staining of mice （×50）； D， Liver weight of mice； E， Liver-to-body weight ratio of mice； F， Liver HE staining of mice （×400）； G， Liver Oil Red O staining of mice （×400）. Each group consisted of 8 mice. Compared with placebo group， *P<0.05， ***P<0.001， nsP>0.05. In fig. C， F and G， the upper and lower panels show two independent biological replicates.