不同毛色豚鼠的TYR及MC1R基因多态性及组织表达分析
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汤银根, 冯亚仙, 钟敏, 卫振, 汪洌, 刘迪文
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Polymorphism and Tissue Expression Analysis of TYR and MC1R Genes in Guinea Pigs with Different Coat-Color Phenotypes
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TANG Yingen, FENG Yaxian, ZHONG Min, WEI Zhen, WANG Lie, LIU Diwen
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表1 测序扩增TYR、MC1R基因编码序列的引物
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Table 1 Primers for amplifying the coding sequences of the TYR and MC1R genes
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引物名称 Primer name | 引物序列（5'→3'） Primer sequence (5' to 3') | 扩增片段长度/bp Length of amplified fragment/bp | 位置 Location |
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TYR-1F1 TYR-1R1 | GCTCAAACAGAGTTTGGAAGCA AAACATGGGAGTTGATCCATTCTT | 534 | 外显子Ⅰ | TYR-1F2 TYR-1R2 | GAGTGTCCCAGAGAAGGATAAATTC ATGAGGTGCTCTGTAAGGACAGATA | 571 | 外显子Ⅰ | TYR-2F TYR-2R | CCTGATTATTAGAAATCAAAGCTGGTAAC AATTTAAACACAAAGTCAGGAGGAAA | 382 | 外显子Ⅱ | TYR-3F TYR-3R | TAGTCATTTGGACTTTTATCAGGTAAACTA AATCCTACCAACTGGGTAAATTAGCATA | 387 | 外显子Ⅲ | TYR-4F TYR-4R | ATATTATGTAGGGCACAATAGCATGAA TAGAGATAACACTCTAGAAACATAAACTT | 462 | 外显子Ⅳ | TYR-5F TYR-5R | AAAAATTGAGATTTCCTTCTATTTCAG GATCTTCACTGACAAACAGGGATT | 365 | 外显子Ⅴ | MC1R-F MC1R-R | CACTCTTGGGACCCCTGGAT TTCCTGCTTTTTGGGGTCATACTC | 1 249 | 外显子 | MC1R-F2 MC1R-R2 | CCCCGTGATAAACAGCACAATGG TTGTGCCTCCTTTGCTTGTGTC | 3 956 or 1 196 | 外显子Ⅰ |
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