SHJH hr 小鼠的心脏衰老表型研究
刘荣乐1, 程灏1, 尚付生2, 常书福1()(), 徐平3
Study on Cardiac Aging Phenotypes of SHJH hr Mice
LIU Rongle1, CHENG Hao1, SHANG Fusheng2, CHANG Shufu1()(), XU Ping3

图4. 24周龄SHJH hr 小鼠的心脏组织氧化损伤相关因子分析
注:A,RT-PCR检测氧化应激因子超氧化物歧化酶(Sod)2、Sod3、谷胱甘肽过氧化物酶(Gpx)1和过氧化氢酶(Cat)的mRNA表达水平;B~D,ELISA法检测SOD、GPX和 CAT活性; E,ELISA法检测8-羟基-2'-脱氧鸟苷(8-OHdG)含量表达;F,ELISA法检测丙二醛(MDA)水平。以非早衰的野生型ICR小鼠(WT组)作为对照,n=6,与WT组比较,*P <0.05。

Figure 4. Analysis of oxidative damage-related factors in cardiac tissue of 24-week-old SHJH hr mice
Note: A, RT-PCR was used to measure the mRNA expression levels ofoxidative stress factors superoxide dismutaseSod2, Sod3, glutathione peroxidaseGpx1, and catalaseCat); B to D, ELISA was used to determine the activities of SOD, GPX, and CAT; E, ELISA was used to measure the expression level of 8-hydroxy-2'-deoxyguanosine (8-OHdG); F, ELISA was used to assess the level of malondialdehyde (MDA). Wild-type ICR mice without premature aging (WT group) were used as controls, n=6. Compared with the WT group, *P <0.05.