SHJH hr 小鼠的心脏衰老表型研究
刘荣乐1, 程灏1, 尚付生2, 常书福1()(), 徐平3
Study on Cardiac Aging Phenotypes of SHJH hr Mice
LIU Rongle1, CHENG Hao1, SHANG Fusheng2, CHANG Shufu1()(), XU Ping3

图3. 24周龄SHJH hr 小鼠的心脏组织病理和相关因子转录水平分析
注：A，HE、Masson 和WGA染色的代表性图片；B，Masson染色显示心肌纤维化水平统计；C，WGA染色显示心肌WGA表达细胞所占面积分析；D，实时荧光定量PCR（RFQ-PCR）检测心肌细胞中Il1β、Il6、Tnf和Ccl2基因的mRNA表达水平；E，RFQ-PCR检测心肌细胞中Tgfβ1、Col1α1和Actα2的mRNA表达水平。以非早衰的野生型ICR小鼠（WT组）作为对照，n=6；与WT组比较，*P <0.05。

Figure 3. Analysis of cardiac histopathology and related factor transcriptional levels in 24-week-old SHJH hr mice
Note： A， Representative images of HE， Masson， and WGA staining； B， Statistical analysis of myocardial fibrosis levels shown by Masson staining； C， Analysis of the area occupied by WGA-positive cells in myocardial tissue as shown by WGA staining； D， Real-time fluorescent quantitative PCR （RFQ-PCR） measurement of mRNA expression levels of Il1β， Il6， Tnf， and Ccl2 in cardiomyocytes； E， RFQ-PCR measurement of mRNA expression levels of Tgfβ1， Col1α1， and Actα2 in cardiomyocytes. Wild-type ICR mice without premature aging （WT group） were used as controls， n=6. Compared with the WT group， *P <0.05.