实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (1): 56-60.DOI: 10.3969/j.issn.1674-5817.2019.01.011

所属专题: 实验动物资源开发与利用

• 经验交流 • 上一篇    下一篇

SPF金黄仓鼠种群的建立及质量控制

张华琼, 夏爽, 吴燕茹, 何凡   

  1. 成都生物制品研究所有限责任公司, 成都 610023
  • 收稿日期:2018-07-27 出版日期:2019-02-25 发布日期:2021-01-29
  • 作者简介:张华琼(1970-),女,硕士,副研究员,主要从事实验动物生产育种和动物实验。E-mail:zhanghuaqiong115@163.com

Establishment and Quality Control of SPF Golden Hamster Colonies

ZHANG Hua-qiong, XIA Shuang, WU Yan-ru, HE Fan   

  1. Chengdu Institute of Biological Products Co. Ltd., Chengdu 610023, China
  • Received:2018-07-27 Online:2019-02-25 Published:2021-01-29

摘要: 目的 为配合乙型脑炎减毒活疫苗世界卫生组织(WHO)预认证,建立高质量的SPF金黄仓鼠种群。方法 通过剖宫产手术按照既定的配种方式建立SPF金黄仓鼠新种群,全面质量控制,定期抽样检测。结果 建立了SPF金黄仓鼠核心群和生产群,19种病毒、6种寄生虫和17种细菌检测阴性。结论 新建SPF金黄仓鼠种群质量符合WHO TRS 980的要求。

关键词: 乙型脑炎活疫苗, 预认证, SPF, 金黄仓鼠, 剖宫产, 质量控制

Abstract: Objective To establish high-quality SPF golden hamster colonies in accordance with WHO pre-certification of live attenuated Japanese Encephalitis Vaccine. Methods A new SPF golden hamster colony was established by caesarean based on the established mating method. The colonies were under general quality control with regular sampling tests. Results The foundation stocks and production stocks of SPF golden hamsters were established, which were negative in 19 virological items、6 parasitological items and 17 bacteriological items. Conclusion A new SPF golden hamster colony is successfully established, the quality meets the requirements of WHO TRS 980.

Key words: Live attenuated japanese encephalitis vaccine, Pre-certification, SPF, Golden hamster, Caesarean, Quality control

中图分类号: