四氯化碳诱导急性肝损伤大鼠模型对心脏的影响

doi:10.12300/j.issn.1674-5817.2021.033

实验动物与比较医学 ›› 2021, Vol. 41 ›› Issue (6): 486-492.DOI: 10.12300/j.issn.1674-5817.2021.033

• 人类疾病动物模型专题 • 上一篇下一篇

四氯化碳诱导急性肝损伤大鼠模型对心脏的影响

张丽丽¹, 万鑫², 冀菁荃¹, 李婷³, 范毅敏⁴, 李宝红¹, 田帅燕⁵

1.山西省长治医学院病理生理学教研室, 长治 046000;
2.山西省长治医学院第一临床学院,长治 046000;
3.山西省长治医学院生理学教研室, 长治 046000;
4.山西省长治医学院机能实验室,长治 046000;
5.山西省长治医学院基础医学部, 长治 046000

收稿日期:2021-02-08 修回日期:2021-06-25 出版日期:2021-12-25 发布日期:2021-12-29
作者简介:张丽丽(1977—), 女, 硕士, 副教授, 主要从事肝硬化相关疾病机制研究。E-mail: LLZ771117@163.com
基金资助:
国家自然科学基金资助项目(81902020); 山西省应用基础研究计划项目(201801D221444)

Effects of Carbon Tetrachloride-induced Acute Liver Injury on Cardiac Pathological Changes in a Rat Model

ZHANG Lili¹, WAN Xin², JI Jingquan¹, LI Ting³, FAN Yimin⁴, LI Baohong¹, TIAN Shuaiyan⁵

1. Department of Pathophysiology, Changzhi Medical College, Changzhi 046000, China;
2. The First Clinical College, Changzhi Medical College, Changzhi 046000, China;
3. Department of Physiology, Changzhi Medical College, Changzhi 046000, China;
4. Functional Integrative Laboratory, Changzhi Medical College, Changzhi 046000, China;
5. Department of Basic Medicine, Changzhi Medical College, Changzhi 046000, China

Received:2021-02-08 Revised:2021-06-25 Online:2021-12-25 Published:2021-12-29

摘要/Abstract

摘要： 目的探讨四氯化碳（CCl₄）对急性肝损伤大鼠心脏功能和形态的影响。方法用低、中、高3种浓度的CCl₄腹腔注射诱导建立急性肝损伤大鼠模型,同时以腹腔注射等体积的植物油作为正常对照组。分别在24 h和48 h行心电图检测,腹主动脉取血后处死动物;用酶联免疫吸附法测定血清丙氨酸转氨酶（alanine aminotransferase,ALT）、心肌肌钙蛋白Ⅰ（cardiac troponinⅠ,cTnⅠ）水平;取肝脏、心脏组织制作切片并行HE染色,光学显微镜观察其病理学改变;用可见光分光光度计测定心肌组织丙二醛（malondialdehyde,MDA）、超氧化物歧化酶（superoxide dismutase,SOD）和肌酸激酶（creatine kinase,CK）含量。结果模型组大鼠血清ALT含量和肝脏形态学的变化均证明CCl₄致急性肝损伤模型制作成功,肝脏损伤程度与CCl₄呈一定的浓度依赖性。心电图检测提示,模型组大鼠心电图出现不同程度的ST段弓背向上抬高、T波高尖、Q-T间期延长等电生理变化。HE染色显示,心脏组织发生明显病理学变化,心肌细胞炎性反应及水肿、坏死程度随CCl₄注射浓度的增加而逐渐加重。血清cTnⅠ水平明显增加（P<0.01）,心肌组织CK、MDA含量明显增加（P<0.05）,SOD含量显著降低（P<0.05）,且各项指标的变化在高浓度CCl₄模型组及诱导后48 h更为显著。结论 CCl₄诱导的急性肝损伤大鼠心脏也发生功能和形态变化,心肌损伤程度呈浓度和时间依赖性。

关键词: 四氯化碳, 急性肝损伤, 心脏病变, 大鼠

Abstract: Objective To investigate the effects of carbon tetrachloride (CCl₄) on cardiac function and morphology in rats with acute liver injury. Methods Rat models of acute liver injury were established by intraperitoneal injection with different concentrations of CCl₄, while an equal volume of vegetable oil was injected intraperitoneally as a control group. Electrocardiogram (ECG) was performed at 24 and 48 hours after inducted by CCl₄, and the animals were sacrificed after blood collection from the abdominal aorta. The levels of serum alanine aminotransferase (ALT) and cardiac troponinⅠ(CTNⅠ) were measured by an enzyme-linked immunosorbent assay. Liver and heart tissues were sectioned and stained with hematoxylin-eosin (HE) and the pathological changes were observed by optical microscopy. The levels of malondialdehyde (MDA), superoxide dismutase (SOD), and creatine kinase (CK) in myocardial tissues were measured by visible light spectrophotometer. Results The changes in serum ALT level and liver morphological in the model groups proved that the acute liver injury model induced by CCl₄ was successfully established, and the degree of liver injury was dose-dependent. The ECG of the model groups revealed electrophysiological changes, such as ST-segment elevation, T-wave sharp, and Q-T interval prolongation in different degrees. HE staining showed that obvious pathological changes in the heart tissue, and the degree of inflammatory reaction, edema and necrosis of myocardial cells gradually aggravated with the increase of CCl₄ injection concentration. Serum CTN Ⅰlevels were significantly increased (P<0.01), CK and MDA levels in the myocardial tissue were significantly increased (P<0.05), while SOD levels in the myocardial tissue were significantly decreased (P<0.05), and the changes in various indexes were more significant in the high concentration CCl₄ group and 48 h after induction by CCl₄. Conclusion CCl₄ can induce cardiac function and morphological changes in rats with acute liver injury, and the degree of myocardial injury is dose- and time-dependent.

Key words: CCl₄, Acute liver injury, Myocardial lesions, Rat

中图分类号:

R363
Q95-33

张丽丽, 万鑫, 冀菁荃, 李婷, 范毅敏, 李宝红, 田帅燕. 四氯化碳诱导急性肝损伤大鼠模型对心脏的影响[J]. 实验动物与比较医学, 2021, 41(6): 486-492.

ZHANG Lili, WAN Xin, JI Jingquan, LI Ting, FAN Yimin, LI Baohong, TIAN Shuaiyan. Effects of Carbon Tetrachloride-induced Acute Liver Injury on Cardiac Pathological Changes in a Rat Model[J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 486-492.

图/表 6

参考文献

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四氯化碳诱导急性肝损伤大鼠模型对心脏的影响

Effects of Carbon Tetrachloride-induced Acute Liver Injury on Cardiac Pathological Changes in a Rat Model

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